Trypsin for Protein Mass Spectrometry

Introducing SOLu-Trypsin: Solution Stable Enzyme for Mass Spectrometry
SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution stable for mass spectrometry. Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation. Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately. SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost. SOLu-Trypsin is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Our highly purified porcine trypsin is available in two forms, under three product listings, to fit the protein chemist’s needs. In both forms, it has been methylated on its lysine residues to prevent autolysis, treated with TPCK (tosyl phenylalanyl chloromethyl ketone) to eliminate chymotryptic activity, and affinity purified. The enzyme comes as either a lyophilized powder (T6567, T7575) or covalently bound to treated silica particles in a convenient spin column (TT0010). The spin column, with its short incubation time of about 15 minutes, is ideal for small volumes. Complete digestion of proteins to tryptic peptides for sequencing or mass spectrometry requires that the protein first be denatured, as folded proteins are usually resistant to proteolysis. Denaturation is commonly accomplished by reduction and methylation, although the use of chaotropic agents such as urea may be more appropriate for some proteins.

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EMS0006 Recombinant Trypsin Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris
EMS0007 Recombinant Trypsin Dimethlyated Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris
EMS0004 SOLu-Trypsin recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
EMS0005 SOLu-Trypsin Dimethylated Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, liquid
T6567 Trypsin from porcine pancreas BioReagent, Proteomics Grade, Dimethylated
T7575 Trypsin Singles, Proteomics Grade
TT0010 Trypsin Spin Columns for proteomics