Chelators and Ion Probes for Microscopy

Microscopy offers great potential for qualitative and quantitative studies on the structure and function of cells and is uniquely suitable for probing living cells. The specimen is illuminated by light of a certain range of wavelengths, which may not necessarily be monochromatic, while viewing emitted (fluorescence) light of longer wavelengths. New methods, like confocal microscopy, provide better resolution (~1.4 improvement) and enable the measurement of various parameters. A point source and a point detector, both achieved by a pinhole are used, while light from other than the focal plane is defocused at the confocal aperture and thus detected only weakly.
Schematic diagram of a confocal microscope

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14510 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid ≥96.0% (HPLC)
14513 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrasodium salt ≥95% (HPLC)
46393 Fluo-3 suitable for fluorescence, ~70%
F0888 Fura 2-AM ≥95% (HPLC)
47989 Fura 2-AM BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
57180 Indo 1 for fluorescence, ≥80.0% (HPLC)
I3261 Indo 1-AM ~95% (HPLC)
65325 8-Methoxypyrene-1,3,6-trisulfonic acid trisodium salt for fluorescence, ≥98.0% (TLC)
72543 Nitr 5/AM suitable for fluorescence, ≥90% (HPLC)
76275 PBFI-AM suitable for fluorescence, ≥98.0% (HPCE)
82658 1,3,6,8-Pyrenetetrasulfonic acid tetrasodium salt hydrate suitable for fluorescence, ≥98% (HPLC)
08520 Quin-2 suitable for fluorescence, ≥95% (HPLC)
29222 Thio-H