Chelators and Ion Probes for Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

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236071 N,N′-Bis(salicylidene)ethylenediamine 98%
C0875 Calcein Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
M8010 N,N′-Dimethyl-9,9′-biacridinium dinitrate used as chemiluminescent reagent
46393 Fluo-3 suitable for fluorescence, ~70%
F0888 Fura 2-AM ≥95% (HPLC)
47989 Fura 2-AM BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
57180 Indo 1 for fluorescence, ≥80.0% (HPLC)
76275 PBFI-AM suitable for fluorescence, ≥98.0% (HPCE)
323497 5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) Dye content 97 %
29222 Thio-H
343633 4,4,4-Trifluoro-1-(2-naphthyl)-1,3-butanedione 99%