Functional Group Reactive Labels for Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

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06627 2-Aminoacridone BioReagent, suitable for fluorescence, ≥98.0% (HPLC)
08445 7-Amino-4-methyl-3-coumarinylacetic acid BioReagent, suitable for fluorescence, ~90% (HPLC)
08658 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt BioReagent, suitable for fluorescence, ≥90% (CE)
09341 8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt BioReagent, suitable for fluorescence, ≥96.0% (HPCE)
69898 Bromobimane BioReagent, suitable for fluorescence, ≥95% (HPCE)
17623 4-Bromomethyl-7-methoxycoumarin for fluorescence, ≥97.0% (HPLC)
21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence, ≥70% (coupling to amines)
30423 3-(Dansylamino)phenylboronic acid BioReagent, suitable for fluorescence, ≥98.0% (HPLC)
39220 Dansyl chloride BioReagent, suitable for fluorescence, ≥99.0% (HPLC)
30434 Dansylhydrazine BioReagent, suitable for fluorescence, ≥90% (HPLC)
34025 Dibromobimane BioReagent, suitable for fluorescence, ≥95.0% (CHN)
36799 7-(Diethylamino)coumarin-3-carboxylic acid BioReagent, suitable for fluorescence, ≥98.0% (HPCE)
36801 7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester BioReagent, suitable for fluorescence, ≥96.0% (HPLC)
38737 6,7-Dimethoxy-4-coumarinylacetic acid BioReagent, suitable for fluorescence, ≥98.0% (TLC)
43095 Diphenylmaleic anhydride suitable for fluorescence, ≥98.0% (HPLC)
46950 Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
46640 7-Fluorobenzofurazan-4-sulfonic acid ammonium salt for HPLC derivatization, ≥98.5% (HPLC)
55156 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)
55634 7-Hydroxy-4-methyl-3-coumarinylacetic acid suitable for fluorescence, ≥98.0% (HPLC)
57676 2-[4-(Iodoacetamido)phenyl]-6-methylbenzothiazole suitable for fluorescence, ≥90% (TLC)
57672 N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid suitable for fluorescence, ≥95% (HPLC)
63184 5-Maleimido-eosin for fluorescence, ≥93% (HPLC)
64948 7-Methoxycoumarin-3-carboxylic acid suitable for fluorescence, ≥97.0% (HPCE)
64949 7-Methoxycoumarin-3-carboxylic acid N-succinimidyl ester suitable for fluorescence, ≥97.0% (HPLC)
64958 2-Methoxy-2,4-diphenyl-3(2H)-furanone suitable for fluorescence, ≥98.0% (HPLC)
68617 10-Methyl-9-(phenoxycarbonyl)acridinium fluorosulfonate ≥90% (HPLC)
69899 Monochlorobimane suitable for fluorescence, ≥70.0% (HPCE)
15167 NIR-797 isothiocyanate suitable for fluorescence, ≥70% (coupling to amines)
78410 o-Phenylenediamine suitable for fluorescence, ≥99.0% (NT)
79760 Phthaldialdehyde for fluorescence, ≥99.0% (HPLC)
82656 N-(1-Pyrenyl)maleimide for fluorescence, ≥99.0% (HPLC)