pH Indicators for Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

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A38401 9-Aminoacridine hydrochloride monohydrate 98%
10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt for fluorescence, ≥97.0% (HPLC)
10419 8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate for fluorescence, ≥95.0% (T)
28605 3-Cyanoumbelliferone BioReagent, suitable for fluorescence, ≥98.0% (TLC)
41525 N,N-Dimethyl-6-propionyl-2-naphthylamine BioReagent, suitable for fluorescence, ≥98.0% (HPLC)
40227 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine suitable for fluorescence, ≥97.0% (HPLC)
55156 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)
55627 7-Hydroxy-4-methyl-2(1H)-quinolone suitable for fluorescence, ≥97.0% (HPLC)
74758 8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt suitable for fluorescence, ≥98.0% (TLC)
P28263 2-Phenylphenol 99%
87917 Tetramethylrhodamine ethyl ester perchlorate suitable for fluorescence, ≥90% (HPCE)
T9792 6-(p-Toluidino)-2-naphthalenesulfonic acid sodium salt