Fluorescent Enzyme Substrates for Microscopy

Microscopy offers great potential for qualitative and quantitative studies on the structure and function of cells and is uniquely suitable for probing living cells. The specimen is illuminated by light of a certain range of wavelengths, which may not necessarily be monochromatic, while viewing emitted (fluorescence) light of longer wavelengths. New methods, like confocal microscopy, provide better resolution (~1.4 improvement) and enable the measurement of various parameters. A point source and a point detector, both achieved by a pinhole are used, while light from other than the focal plane is defocused at the confocal aperture and thus detected only weakly.
Schematic diagram of a confocal microscope

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16670 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt ≥95.0% (HPLC)
21884 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)
37291 Dihydroethidium BioReagent, suitable for fluorescence, ≥95% (HPCE)
F2256 Fast Red RC Salt
46943 Fluorescein dilaurate suitable for fluorescence, ≥97.0% (HPCE)
M2405 4-Methylumbelliferyl β-D-lactopyranoside β-galactosidase substrate
P6787 o-Phenylenediamine dihydrochloride tablet, 2 mg substrate per tablet
P3804 o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
P6912 o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
P8287 o-Phenylenediamine dihydrochloride tablet, 10 mg substrate per tablet
P8412 o-Phenylenediamine dihydrochloride tablet, 30 mg substrate per tablet
83636 Resorufin acetate suitable for fluorescence, ≥98.0% (TLC)