Fluorescent Enzyme Substrates for Immunoassays

General Features & Benefits
  • Immunological methods are based upon the competitive binding of labeled ligands versus unlabeled ones on highly specific receptor sites
  • Very important tools for clinical and analytical biochemistry and the analysis of drugs, pesticides, hormones and proteins
  • Outstanding specificity and sensitivity, adaptability to automation & an ability to quantitate an almost limitless number of analytes.
  • Competitive (higher selectivity) and non-competitive (higher sensitivity) immunoassay design

Heterogeneous Immunoassays
  • Physical separation of bound from free labeled analyte. Mostly the analyte or antibody is attached to a solid surface
  • Detection can be direct (only a primary antibody is used) or indirect (a secondary antibody is also used)

Homogenous Immunoassays
  • Fluorescence polarization excitation of fluorochromes
  • Double-antibody fluorophore-labeled antigen reaction with antibodies directed against both the antigen and the fluorophore. Labeled and unlabeled antigens compete for a limited number of anti-antigen antibodies

Product #


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A80602 5-Aminotetrazole monohydrate 97%
A9941 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt tablet, 10 mg substrate per tablet
11557 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ≥98.0% (HPLC)
11585649910 Biotin-High Prime
33430 o-Dianisidine for spectrophotometric det. of Au, NO2-, Ce(IV), for the detection of Au, Co, Cu, SCN-, V, ≥97.0%
EHIFI-RO Expand High Fidelity PCR System
EHIFIDNTP-RO Expand High Fidelity PCR System, dNTPack
EHIFIP-RO Expand High FidelityPLUS PCR System
EHIFIPN-RO Expand High FidelityPLUS PCR System, dNTPack
11811002001 Expand 20 kbPLUS PCR System
ELONGN-RO Expand Long Range dNTPack
FHIFI-RO FastStart High Fidelity PCR System
FHIFIN-RO FastStart High Fidelity PCR System, dNTPack
FPCR-RO FastStart PCR Master
FTAQ-RO FastStart Taq DNA Polymerase, 5 U/μl
FPCRN-RO FastStart Taq DNA Polymerase, dNTPack
12140306001 GC-RICH PCR System
12140314001 High Fidelity PCR Master
11666649001 Hybridization Bags
70448 2-Naphthol BioXtra, ≥99.0% (GC)
71768 4-Nitrophenyl phosphate disodium salt hexahydrate for enzyme immunoassay, ≥99.0% (enzymatic)
11699075001 Nylon Membranes for Colony and Plaque Hybridization
NYLM-RO Nylon Membranes, positively charged
P8287 o-Phenylenediamine dihydrochloride tablet, 10 mg substrate per tablet
P8412 o-Phenylenediamine dihydrochloride tablet, 30 mg substrate per tablet
P6912 o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
P3804 o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
P6787 o-Phenylenediamine dihydrochloride tablet, 2 mg substrate per tablet
87748 3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)
87750 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate ≥98.0% (calc. on dry substance, AT)