Fluorescent Enzyme Substrates for Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

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A4302 L-Alanine 7-amido-4-methylcoumarin trifluoroacetate salt aminopeptidase substrate
A8264 4-Aminophthalhydrazide
E1379 7-Ethoxycoumarin
46943 Fluorescein dilaurate suitable for fluorescence, ≥97.0% (HPCE)
H52406 3-(4-Hydroxyphenyl)propionic acid 98%
64951 7-Methoxycoumarin suitable for fluorescence, ≥98.0% (TLC)
220337 7-Methoxycoumarin ≥98%
69587 2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid sodium salt hydrate BioReagent, suitable for fluorescence, ≥96.5% (HPLC)
69585 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide suitable for fluorescence, ≥99.0% (TLC)
19362 4-Methylumbelliferyl butyrate suitable for fluorescence, ≥95% (HPCE)
69591 4-Methylumbelliferyl α-D-glucopyranoside for fluorescence, ≥99% (TLC)
69602 4-Methylumbelliferyl-β-D-glucuronide hydrate suitable for fluorescence, ≥99.0% (HPLC)
51010 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate ≥85% (HPLC)
M2405 4-Methylumbelliferyl β-D-lactopyranoside β-galactosidase substrate
75164 4-Methylumbelliferyl oleate suitable for fluorescence, ≥95% (HPCE)
70448 2-Naphthol BioXtra, ≥99.0% (GC)
74875 8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt suitable for fluorescence, ≥90% (HPCE)
83636 Resorufin acetate suitable for fluorescence, ≥98.0% (TLC)
83637 Resorufin butyrate suitable for fluorescence, ≥95% (TLC)
46121 Resorufin ethyl ether suitable for fluorescence, ≥95% (UV)
R4758 Resorufin β-D-glucopyranoside ≥90%
P0928 Resorufin pentyl ether
96286 Z-Gly-Pro-4-nitroanilide ≥99.0% (TLC)