Individual Protease Inhibitors

Don't Lose Your Proteins During Sample Prep

Protease inhibitors are tremendously valuable and useful reagents for researchers who want to inhibit general degradation of proteins in tissue or cell extracts by endogenous proteases, or to investigate particular processes that involve blocking the activity of specific proteases.

Four main classes of proteolytic enzymes have been routinely utilized to describe proteases. The serine proteases are probably the best characterized. This class of proteases includes trypsin, chymotrypsin and elastase. The cysteine protease class includes papain, calpain and lysosomal cathepsins. Aspartic proteases include pepsin and rennin. Metallo-proteases include thermolysin and carboxypeptidase A.

During isolation and characterization, one or all four classes of proteases may pose a threat to the fate of a protein. Broad spectrum protease inhibitors and mixtures, or cocktails, have been developed to help protect the integrity of proteins.

Sigma-Aldrich offers its well-established individual protease inhibitors, along with new individual protease inhibitors from Roche, for situations when you need a single protease inhibitor rather than a protease inhibitor cocktail to protect your proteins during sample prep.

  • Widest selection of protease inhibitors for broad spectrum coverage against proteases of any supplier
  • Inhibits proteases in almost any tissue or cell
  • Specific formulations optimized for particular applications

Do you want to create your own protease inhibitor mixture? View our Broad Spectrum Inhibitors of Proteolytic Enzyme Classes.

Product #



CAS Number

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5.30616 ACE2 Inhibitor, MLN-4760 ≥97% (HPLC), powder, Calbiochem® 305335-31-3 428.3
A1276 Amastatin hydrochloride hydrate ≥97% (HPLC) 100938-10-1 511
A6664 4-Amidinophenylmethanesulfonyl fluoride hydrochloride serine protease inhibitor 74938-88-8 252.7
06880 4-Aminobenzamidine dihydrochloride BioReagent, suitable for fluorescence, ≥99.0% (TLC) 2498-50-2 208.1
A8456 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride ≥97.0% (HPLC) 30827-99-7 239.7
A6191 Antipain dihydrochloride from microbial source protease inhibitor 37682-72-7 677.6
A9024   α1-Antitrypsin from human plasma salt-free, lyophilized powder 9041-92-3  
A1153 Aprotinin from bovine lung lyophilized powder, 3-8 TIU/mg solid 9087-70-1 6511.4
A6279 Aprotinin from bovine lung saline solution, 3-7 TIU/mg protein 9087-70-1 6511.4
A3428 Aprotinin from bovine lung lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture 9087-70-1 6511.4
A4529 Aprotinin from bovine lung lyophilized powder, 3-7 TIU/mg solid 9087-70-1 6511.4
B8385 Bestatin hydrochloride ≥98% (HPLC) 65391-42-6 344.8
D216305 2,2′-Bipyridyl ReagentPlus®, ≥99% Green Alternative 366-18-7 156.2
C7268 Chymostatin microbial 9076-44-2  
C8917   Cystatin from chicken egg white lyophilized powder 81989-95-9  
C0408   Cystatin from chicken egg white buffered aqueous glycerol solution 81989-95-9  
D7910 3,4-Dichloroisocoumarin serine protease inhibitor 51050-59-0 215
D0879 Diisopropylfluorophosphate 55-91-4 184.2
E3132 E-64 protease inhibitor 66701-25-5 357.4
E64-RO E-64 N-[N-(L-3-Trans-carboxirane-2-carbonyl)-L-leucyl]-agmatine 66701-25-5 357.4
E8640 E-64d protease inhibitor 88321-09-9 342.4
L2884 Leupeptin microbial, ≥90% (HPLC) 103476-89-7 475.6
LEU-RO Leupeptin Ac-Leu-Leu-argininal x 1/2 H2SO4, synthetic 103476-89-7 475.6
L8511 Leupeptin synthetic, ≥85% (HPLC) 103476-89-7 475.6
L5793 Leupeptin BioUltra, microbial, ≥95% (HPLC) 103476-89-7 475.6
L9783 Leupeptin hydrochloride microbial, ≥90% (HPLC) 24125-16-4 463
L0649 Leupeptin hydrochloride microbial, ≥70% (HPLC) 24125-16-4 463
L2023 Leupeptin trifluoroacetate salt ≥90% (HPLC), microbial 147385-61-3 426.6
L7386 Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein 9011-93-2  
M6159 α2-Macroglobulin from human plasma BioUltra, ≥98% (SDS-PAGE) 1631137-21-7  
PEFBSC-RO Pefabloc® SC    
PEPS-RO Pepstatin from Streptomyces species 26305-03-3 685.9
P5318 Pepstatin A microbial, ≥90% (HPLC) 26305-03-3 685.9
P4265 Pepstatin A microbial, ≥75% (HPLC) 26305-03-3 685.9
P9375 1,10-Phenanthroline monohydrate reagent grade 5144-89-8 198.2
P7626 Phenylmethanesulfonyl fluoride ≥98.5% (GC) 329-98-6 174.2
R7385 Phosphoramidon disodium salt ≥97% (HPLC) 119942-99-3 587.5
A8111   Plasminogen activator inhibitor 1 (PAI-1) human recombinant, expressed in E. coli, ≥90% (SDS-PAGE) 140208-23-7  
PMSF-RO PMSF Phenylmethylsulfonyl fluoride 329-98-6 174.2
INHIB1   Protease Inhibitor Panel for custom cocktail preparation    
11206893001 Protease Inhibitors Set    
T7254 Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride ≥96% (TLC), powder 4272-74-6 369.3
T4376 N-p-Tosyl-L-phenylalanine chloromethyl ketone ≥97% (TLC), powder 402-71-1 351.9
10109886001   Trypsin Inhibitor from soybean    
10109878001   Trypsin Inhibitor from chicken egg white    
T2011 Trypsin inhibitor from chicken egg white Type III-O (free of ovoinhibitor) 9035-81-8  
T9003 Trypsin inhibitor from Glycine max (soybean) lyophilized powder 9035-81-8  
T2327 Trypsin Inhibitor from Glycine max (soybean) BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE) 9035-81-8