ChIP Validated Antibodies

Successful chromatin immunoprecipitation (ChIP) requires an effective, specific, and high quality antibody. Not all antibodies work in ChIP as the antigen epitope may be blocked or altered once cross-linked. Sigma′s Imprint brand of antibodies are validated in ChIP application and lot tested each time for successful chromatin immunoprecipitation experiment.

Chromatin immunoprecipitation (ChIP) is a powerful tool for identifying proteins, including histone proteins and non-histone proteins, associated with specific regions of the genome by using specific antibodies that recognize a specific protein or a specific modification of a protein. The ChIP protocol is based on the principle that formaldehyde reacts with primary amines located on amino acids and the bases on DNA or RNA molecules, forming a covalent crosslink between the specific protein to the DNA on which they are situated. Once the DNA binding proteins are crosslinked, the cells are lysed and the crude cell extracts are sonicated to shear the DNA to a smaller size. The protein:DNA complex is immunoprecipitated using an antibody against the protein of interest. The DNA protein cross-links are reversed by heating and the proteins removed by treatment with proteinase K. The DNA portion of the complex is then purified and identified by PCR using specific primers to the suspected binding region.

Product #

Description

Form

Gene Symbol (ID)

Application

Add to Cart

17-10032 ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonal     cell analysis, ChIP, DB, IP, WB
17-10034 ChIPAb+ EED - ChIP Validated Antibody and Primer Set   human ... EED(8726)
ChIP, IP, WB
17-10044 ChIPAb+ CTCF - ChIP Validated Antibody and Primer Set   human ... CTCF(10664)
ChIP, DB, ELISA, IHC, IP, multiplexing, WB
17-10045 ChIPAb+ Acetyl-Histone H4 (Lys5) - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
  ChIP, IP, WB
17-10046 ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
  ChIP, DB, IP, WB
17-10047 ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal     ChIP, IP, WB
17-10050 ChIPAb+ Acetyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-10054 ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-10057 ChIPAb+ SMRT - ChIP Validated Antibody and Primer Set ascites fluid
human ... NCOR2(9612), TRAC(28755)
ChIP, EMSA, ICC, IHC, IP, WB
17-10060 ChIPAb+ NFκB p65 (RelA) - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-10061 ChIPAb+ E2F-1 - ChIP Validated Antibody and Primer Set     ChIP, IP
17-10098 ChIPAb+ TATA Binding Protein (TBP) - ChIP Validated Antibody and Primer Set ascites fluid
human ... TBP(6908)
ChIP, IP, WB
17-10099 ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set serum
  ChIP, IP, WB
17-10101 ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-10108 ChIPAb+ Dimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
  cell analysis, ChIP, IP, WB
17-10109 ChIPAb+ TCF-4 - ChIP Validated Antibody and Primer Set   human ... TCF7L2(6934)
ChIP, DB, IP, WB
17-10111 ChIPAb+ Acetyl-Histone H3 (Lys18) - ChIP Validated Antibody and Primer Set serum
  ChIP, DB, WB
17-10112 ChIPAb+ Acetyl Histone H3 (Lys23) - ChIP Validated Antibody and Primer Set serum
  ChIP, IP, WB
17-10121 ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal     cell analysis, ChIP, WB
17-10125 ChIPAb+ Dimethyl-Histone H3 (Lys79) - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
human ... H3F3B(3021)
ChIP, WB
17-10130 ChIPAb+ Trimethyl-Histone H3 (Lys79) - ChIP Validated Antibody and Primer Set     ChIP, multiplexing, WB
17-10131 ChIPAb+ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set   human ... CREB1(1385)
ChIP, EMSA, IHC, IP, WB
17-10135 ChIPAb+ CBX8 - ChIP Validated Antibody and Primer Set     ChIP, WB
17-10136 ChIPAb+ Dimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set serum
  ChIP, EMSA, IP, WB
17-10141 ChIPAb+ Phospho-Histone H3 (Thr3) - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
  ChIP, DB, WB
17-10199 ChIPAb+ HDAC1 Antibody, rabbit polyclonal     ChIP, ICC, WB
17-10237 ChIPAb+ HDAC2 - ChIP Validated Antibody and Primer Set   human ... HDAC2(3066)
ChIP, WB
17-10241 ChIPAb+ Acetyl-Histone H3 (Lys9/18) - ChIP Validated Antibody and Primer Set serum
human ... H3F3B(3021)
ChIP, WB
17-10242 ChIPAb+ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set     ChIP, WB
17-10245 ChIPAb+ Histone H3.3 - ChIP Validated Antibody and Primer Set   human ... H3F3B(3021)
ChIP, DB, WB
17-10250 ChIPAb+ Dimethyl-Histone H4 (Arg3) Symmetric - ChIP Validated Antibody and Primer Set     ChIP, DB, WB
17-10254 ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set serum
  ChIP, DB, ICC, inhibition assay, WB
17-10258 ChIPAb+ FOXA2 - ChIP Validated Antibody and Primer Set     ChIP, ICC, WB
17-10259 ChIPAb+ Acetyl-Histone H3 (Lys56) - ChIP Validated Antibody and Primer Set   human ... HIST1H3F(8968)
ChIP, ICC, WB
17-10260 ChIPAb+ N-CoR Antibody     ChIP, WB
17-10262 ChIPAb+ JMJD1C - ChIP Validated Antibody and Primer Set   human ... JMJD1C(221037)
ChIP, ICC, WB
17-10263 ChIPAb+ JMJD6 - ChIP Validated Antibody and Primer Set     ChIP, WB
17-10264 ChIPAb+ JHDM1B Antibody   human ... KDM2B(84678)
ChIP, DB, WB
17-10267 ChIPAb+ FOXA1 Antibody   human ... FOXA1(3169)
ChIP, ICC, WB
17-10269 ChIPAb+ Phospho-Histone H3 (Ser28) Antibody     ChIP, DB, ICC, inhibition assay, WB
17-10456 ChIPAb+ REST - ChIP Validated Antibody and Primer Set     ChIP, WB
17-10489 ChIPAb+ Androgen Receptor- ChIP Validated Antibody and Primer Set     ChIP, IHC, IP, WB
17-10491 ChIPAb+ MeCP2 - ChIP Validated Antibody and Primer Set   human ... MECP2(4204)
ChIP, IP, WB
17-10493 ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set     ChIP, DB, IP, WB
17-10498 ChIPAb+ Monomethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set     ChIP, DB, IP, WB
17-10530 ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set     ChIP, WB
17-10531 ChIPAb+ LSD1 (KDM1A) Antibody - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-600 ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
human ... CREB1(1385)
ChIP, WB
17-601 ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set     ChIP, WB
17-603 ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set ascites fluid
human ... ESR1(2099)
ChIP, WB
17-604 ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set   human ... LEF1(51176)
ChIP, WB
17-608 ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set culture supernatant
  ChIP, WB
17-609 ChIPAb+ Acetyl-Histone H3 (Lys9) Serum - ChIP Validated Antibody and Primer Set serum
human ... H3F3B(3021)
ChIP, IP, WB
17-613 ChIPAb+ p53 - ChIP Validated Antibody and Primer Set     ChIP, WB
17-614 ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal   human ... H3F3B(3021)
ChIP, WB
17-615 ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set     ChIP, WB
17-620 ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set     ChIP, IF, IP
17-622 ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set   human ... H3F3B(3021)
ChIP, WB
17-625 ChIPAb+ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set   human ... H3F3B(3021)
ChIP, DB, WB
17-630 ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set serum
  cell analysis, ChIP, IP, multiplexing, WB
17-643 ChIPAb+ Monomethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set serum
human ... H3F3B(3021)
ChIP, EMSA, FACS, IF, IHC, IP, WB
17-646 ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set ascites fluid
human ... CBX3(11335)
cell analysis, ChIP, IP, WB
17-648 ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set serum
human ... H3F3B(3021)
ChIP, IP, WB
17-650 ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set     ChIP, IF, IP, WB
17-651 ChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set     ChIP, WB
17-655 ChIPAb+ Nanog Antibody - ChIP Validated Antibody and Primer Set     ChIP, ICC, WB
17-656 ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set     ChIP, DB, ICC, IP, WB
17-658 ChIPAb+ Acetyl-Histone H3 (Lys9) Purified - ChIP Validated Antibody and Primer Set   human ... H3F3B(3021)
ChIP, ICC, IP, WB
17-661 ChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set   human ... SUZ12(23512)
ChIP, DB, IP, WB
17-662 ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set   human ... EZH2(2146)
ChIP, WB
17-663 ChIPAb+ EED - ChIP Validated Antibody and Primer Set   human ... EED(8726)
ChIP, IP, WB
17-671 ChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set, rabbit monoclonal culture supernatant
  ChIP, IP, WB
17-675 ChIPAb+ Histone H3 (Unmodified Lys4) - ChIP Validated Antibody and Primer Set     ChIP, WB
17-676 ChIPAb+ Monomethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set     ChIP, WB
17-677 ChIPAb+ Dimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set     ChIP, DB, multiplexing, WB
17-678 ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set     ChIP, ICC, WB
17-680 ChIPAb+ Monomethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-681 ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set     ChIP, WB
17-683 ChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set     ChIP, IP, WB
17-685 ChIPAb+ Phospho-Histone H3 (Ser10) - ChIP Validated Antibody and Primer Set     ChIP, WB
SAB4800002 Imprint® Monoclonal Anti-5-hydroxymethylcytosine antibody produced in rat affinity isolated antibody
  ELISA (i), methylated DNA immunoprecipitation (MeDIP)
SAB4800006 Imprint® Anti-H3K36me3 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800010 Imprint® Anti-H3K4me1, (N-terminal) antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800011 Imprint® Monoclonal Anti-TBP, (N-terminal) antibody produced in mouse purified antibody
human ... TBP(6908)
ChIP, IHC (p)
SAB4800013 Imprint® Anti-TIP5 antibody produced in rabbit whole antiserum
human ... BAZ2A(11176)
ChIP, WB
SAB4800014 Imprint® Anti-HDAC1, (C-terminal) antibody produced in rabbit affinity isolated antibody
human ... HDAC1(3065)
ChIP, ELISA (i), WB
SAB4800019 Imprint® Monoclonal Anti-HDAC1, (C-terminal) antibody produced in mouse affinity isolated antibody
human ... HDAC1(3065)
ChIP, WB
SAB4800023 Imprint® Anti-H3S10p antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), IF (d), WB
SAB4800024 Imprint® Anti-H4Pan antibody produced in rabbit affinity isolated antibody
human ... H4F3(3023)
ChIP, ELISA (i)
SAB4800029 Imprint® Anti-H3K79me2 antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800033 Imprint® Anti-H3K27me3S28p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), IF (d), IP, WB
SAB4800035 Imprint® Anti-H4K8ac antibody produced in rabbit whole antiserum
human ... H4F3(3023)
ChIP, ELISA (i), WB
SAB4800036 Imprint® Anti-H3S10p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), IF (d), IP, WB
SAB4800038 Imprint® Anti-H3R17me2 antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800040 Imprint® Monoclonal Anti-ERa antibody produced in mouse purified antibody
human ... ESR1(2099)
ChIP, IHC (p), WB
SAB4800042 Imprint® Anti-H2Bpan antibody produced in rabbit affinity isolated antibody
human ... H2BFM(286436)
ChIP, ELISA (i)
SAB4800043 Imprint® Anti-H3K79me1 antibody produced in rabbit affinity isolated antibody
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800044 Imprint® Anti-H3K9acS10p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800045 Imprint® Anti-H3K9me3S10p antibody produced in rabbit whole antiserum
human ... H3F3A(3021), H3F3B(3021)
ChIP, ELISA (i), WB
SAB4800046 Imprint® Anti-H4K8ac antibody produced in rabbit purified antibody
human ... H4F3(3023)
ChIP, ELISA (i), WB
SAB5100001 Imprint® ChIP-Validated Monoclonal Anti-p53 (DO-1) antibody produced in mouse purified immunoglobulin
human ... TP53(7157)
ChIP, WB
SAB5100002 Imprint® ChIP-Validated Monoclonal Anti-p53 (DO-7) antibody produced in mouse purified immunoglobulin
human ... TP53(7157)
ChIP, WB
SAB5100003 Imprint® ChIP-Validated Monoclonal Anti-p53 (PAb1801) antibody produced in mouse purified immunoglobulin
human ... TP53(7157)
ChIP, WB
SAB5100004 Imprint® ChIP-Validated Monoclonal Anti-p53 (BP53-12) antibody produced in mouse ascites fluid
  ChIP, WB
SAB5100005 Imprint® ChIP-Validated Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse purified immunoglobulin
human ... POU5F1(5460)
ChIP, WB