Antibodies to Modifying Enzymes/Histone Deacetylase
Regulation of gene expression is mediated by several mechanisms including DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones. One of the major modifications of histones consists of the dynamic acetylation and deacetylation of ε-amino groups of lysine residues present in the tail of core histones.
1 The enzymes responsible for this reversible acetylation/deacetylation process are histone acetyltransferases (HATs) and histone deacetylases (HDACs), respectively.
3 Mammalian HDACs can be divided into three classes according to sequence homology.
4 Class I consists of the yeast Rpd3-like proteins HDAC1, HDAC2, HDAC3 and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10.
5 Class III comprises the yeast Sir2-like proteins. Whereas class I HDACs are ubiquitously expressed, most class II HDACs are tissue-specific. Sir2 is an NAD-dependent histone deacetylase that appears to play an important role in chromatin silencing and life span prolongation through calorie restriction.
6 Sigma
® has a wide selection of antibodies to many of the histone modifying enzymes. The antibodies are carefully tested to provide high quality performance. These antibodies are valuable tools for scientists working on chromatin remodeling.
References 1. Wang, A.H., et al. Mol. Cell. Biol.,
19 , 7816-7827 (1999).
2. Grozinger, C.M. et al. Proc. Natl. Acad. Sci. USA,
96 , 4868-4873 (1999).
3. Fischle, W., et al. Biochem. Cell Biol.,
79 , 337 (2001).
4. Khochbin, S., et al. Curr. Opin. Genet. Dev.,
11 , 162-166 (2001).
5. Fischle, W., et al. J. Biol. Chem.,
274 , 11713-11720 (1999).
Product #
Description
Clone
Form
Gene Symbol (ID)
Species Reactivity
Application
Add to Cart
HPA029693
Anti-HDAC1 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC1 (3065)
human
IF, IHC
WH0055869M1
Monoclonal Anti-HDAC8 antibody produced in mouse
2F4, monoclonal
purified immunoglobulin
human ... HDAC8 (55869)
mouse, human, rat
ELISA (i), IF, WB
HPA028926
Anti-HDAC9 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC9 (9734)
human
IF, IHC
H3284
Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit
polyclonal
IgG fraction of antiserum
human ... HDAC1 (3065) mouse ... Hdac1 (433759)
human, mouse
ARR, IHC (p), IP, WB
H3159
Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit
polyclonal
IgG fraction of antiserum
human ... HDAC2 (3066) mouse ... Hdac2 (15182)
mouse, human
ARR, IHC (p), IP, WB
H3034
Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit
polyclonal
IgG fraction of antiserum
human ... HDAC3 (8841) mouse ... Hdac3 (15183) rat ... Hdac3 (84578)
human, rat, mouse
ARR, IHC (p), IP, WB
H6537
Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse
HDAC3-83, monoclonal
purified immunoglobulin
human ... HDAC3 (8841) mouse ... Hdac3 (15183)
mouse, human
ARR, WB
H9536
Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC4 (9759) mouse ... Hdac4 (208727) rat ... Hdac4 (363287)
rat, mouse, human
ARR, IF, IP, WB
H9411
Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC4 (9759) mouse ... Hdac4 (208727) rat ... Hdac4 (363287)
rat, mouse, human
ARR, IF, IP, WB
H8163
Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC5 (10014) mouse ... Hdac5 (15184) rat ... Hdac5 (84580)
rat, mouse, human
ARR, ICC, IHC, IP, WB CL
H2287
Anti-Histone Deacetylase 6 (HDAC6) antibody produced in rabbit
polyclonal
IgG fraction of antiserum
human ... HDAC6 (10013) mouse ... Hdac6 (15185)
human, mouse
ARR, IP, WB
H2662
Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC7 (51564) mouse ... Hdac7 (56233) rat ... Hdac7a (84582)
human, mouse, rat
IF, IP, WB
H2537
Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC7 (51564) mouse ... Hdac7 (56233) rat ... Hdac7a (84582)
mouse, rat, human
ARR, IP, WB
H8038
Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC8 (55869) mouse ... Hdac8 (70315)
human, mouse
ICC, IP, WB
H6412
Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse
HDAC8-48, monoclonal
purified immunoglobulin
human ... HDAC8 (55869)
human
ARR, ELISA (i), WB
H3413
Anti-Histone Deacetylase 10 (HDAC10) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC10 (83933) mouse ... Hdac10 (170787) rat ... Hdac10 (362981)
human, rat, mouse
ARR, IF, WB CL
H4539
Anti-Histone Deacetylase 11 (HDAC11) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... HDAC11 (79885) mouse ... Hdac11 (232232) rat ... Hdac11 (297453)
mouse, human
WB CL
H6287
Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal
HDAC1-21, monoclonal
purified immunoglobulin
human ... HDAC1 (3065) mouse ... Hdac1 (433759)
mouse, human
ARR, ELISA (i), IP, WB
H2663
Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal
HDAC2-62, monoclonal
purified immunoglobulin
human ... HDAC2 (3066) mouse ... Hdac2 (15182) rat ... Hdac2 (84577)
rat, canine, human, chicken, mouse, bovine
ARR, ELISA (i), IHC, IP, WB
H0163
Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal
HDAC4-144, monoclonal
purified from hybridoma cell culture
human ... HDAC4 (9759) mouse ... Hdac4 (208727) rat ... Hdac4 (363287)
human, mouse, rat
ICC, IP, WB
H4538
Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal
HDAC5-35, monoclonal
purified from hybridoma cell culture
human ... HDAC5 (10014) mouse ... Hdac5 (15184) rat ... Hdac5 (84580)
mouse, rat, human
ARR, ELISA (i), ICC, IP, WB
H6663
Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal
HDAC7-97, monoclonal
purified from hybridoma cell culture
human ... HDAC7 (51564) mouse ... Hdac7 (56233)
human, mouse
ARR, ELISA (i), ICC, IP, WB
S5313
Anti-Sir2 (AS-16) antibody produced in rabbit
polyclonal
affinity isolated antibody
mouse ... Sirt1 (93759)
mouse
ARR, IF, WB
S5447
Anti-SIRT1 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... SIRT1 (23411) mouse ... Sirt1 (93759) rat ... Sirt1 (309757)
human
IP, WB
S5196
Anti-Sirt1 antibody, Mouse monoclonal
SIR11, monoclonal
purified from hybridoma cell culture purified immunoglobulin
mouse ... Sirt1 (93759)
mouse
ARR, ELISA (i), WB
S5322
Anti-Sirt1 (C-terminal) antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... SIRT1 (23411) mouse ... Sirt1 (93759) rat ... Sirt1 (309757)
human
IF, IP, WB
SAB2500945
Anti-SIRT3 antibody produced in goat
polyclonal
affinity isolated antibody
human ... SIRT3 (23410)
human
ELISA (i), WB
S4072
Anti-Sirt3 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... SIRT3 (23410)
human
IF, WB
HPA026809
Anti-SIRT3 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... SIRT3 (23410)
human
IHC
AV32388
Anti-SIRT3 antibody produced in rabbit
polyclonal
IgG fraction of antiserum
human ... SIRT3 (23410)
horse, human, mouse, rat, dog
WB
S5947
Anti-Sirt7 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... SIRT7 (51547) mouse ... Sirt7 (209011)
human, mouse (predicted)
IF, IP, WB
AV32406
Anti-SIRT7 antibody produced in rabbit
polyclonal
affinity isolated antibody
human ... SIRT7 (51547)
dog, bovine, rat, mouse, human, pig, horse
WB