Purified Immunoglobulins

A wide range of human and animal immunoglobulins (Igs) for use in immunological and biochemical applications have been isolated from normal or myeloma sources. Purified Igs may be used in various immunoassays and biochemical applications as positive or negative controls, standards, coating antigens, or blocking agents. They may also serve as starting material for the preparation of immunogens, tolerogens, or solid-phase immunosorbents. Our high standards and rigorous testing procedures ensure a high level of product quality, purity, and consistency. Normal Igs are offered in two grades, Reagent Grade and Technical Grade, to meet a wide range of customer needs.
Reagent Grade
Reagent Grade Igs are prepared from normal sources, such as the serum of animals that have not been immunized with a specific protein. A combination of fractionation, ion-exchange chromatography and/or gel filtration chromatography is used to isolate the Igs. IgG and IgM are prepared from pooled normal serum. IgA is prepared from normal colostrum and, therefore, is in the secretory form.
Reagent grade Igs have been prepared to meet the highest purity standards. They are determined to be > 95% pure by non-reducing SDS-PAGE.
The high purity of Reagent Grade Igs makes them excellent reagents for sensitive immunoassays. They may be used as:
  • Standards
  • Blocking agents
  • Negative controls

They are also suitable starting materials for:
  • Immunogens for the production of antiserum
  • Tolerogens to induce tolerance to a specific class of immunoglobulin
  • Conjugation to solid supports to prepare immunosorbents for the purification or removal of antibodies and other proteins with a specificity for the individual Igs.
Technical Grade
Technical Grade Igs are isolated from pooled normal serum by fractionation. Their purity is determined to be > 80% by non-reducing SDS-PAGE.
Technical Grade Igs are an economical alternative for applications that do not require the high level of purity of the Reagent Grade Igs. They may be used as negative control antibodies when the experimental antibody is in the form of an IgG fraction (i.e. not as pure as an affinity isolated antibody). They may also serve as blocking agents in immunoassays or as starting material for procedures involving downstream purification such as papain or pepsin digestions to produce Fc, Fab, or F(ab)2 fragments of IgG.