Reagents For Analyzing Autophagy

The vital dyes Acridine Orange and Dansylcadaverine (MDC) are commonly used to study autophagy. Acridine Orange is a lysotropic dye that accumulates in acidic organelles in a pH-dependent manner. At neutral pHs, Acridine Orange is a hydrophobic green fluorescent molecule. Within acidic vesicles, Acridine Orange becomes protonated and trapped within the organelle. Protonated Acridine Orange forms aggregates that emit bright red fluorescence. MDC is another popular autofluorescent marker that preferentally accumulates in autophagic vacuoles. While Acridine Orange staining in lysosomes is primarily due to ion trapping, MDC accumulation in auotphagic vacuoles is due to a combination of ion trapping and specific interactions with vacuole membrane lipids.

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318337 Acridine Orange hydrochloride hydrate ≥98% (HPLC)
A8097 Acridine Orange hydrochloride solution 10 mg/mL in H2O
D4008 Dansylcadaverine ≥97% (TLC)
30432 Dansylcadaverine BioReagent, suitable for fluorescence, ≥99.0% (HPLC)
L7543 Anti-LC3B antibody produced in rabbit ~1 mg/mL, affinity isolated antibody, buffered aqueous solution