Histopaque®

Histopaque® iodinated gradient media

Iodinated aromatic compounds, originally devised for x-ray contrast applications, solve the more serious deficiencies of other classes of gradient media. Iodinated gradient media have much lower osmolarities and viscosities than sucrose at any concentration. Polysaccharides, such as Ficoll®, are even more viscous than sucrose at all densities. Ionic gradient media, such as cesium chloride, have higher densities and lower viscosities than other density gradient media. However, their use is restricted due to the high osmolarities and ionic strength which affect the hydration of osmotically sensitive particles and can disrupt or otherwise modify the integrity of biological particles.
Because of their positive properties, iodinated gradient media are used in a wide range of applications. Their structures are usually based on tri-iodobenzoic acid with a hydrophilic group attached to increase solubility.
Histopaque® media are sterile, endotoxin-tested solutions of polysucrose and sodium diatrizoate, adjusted to precise densities. These ready-to-use separation media facilitate the rapid and optimal recovery of viable cells from small volumes of whole blood.

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10771 Histopaque®-1077 sterile-filtered, density: 1.077 g/mL
H8889 Histopaque®-1077 Hybri-Max liquid, sterile-filtered, BioReagent, suitable for hybridoma
10831 Histopaque®-1083 sterile-filtered, density: 1.083 g/mL
11191 Histopaque®-1119 sterile-filtered, density: 1.119 g/mL