Polyhydric alcohols

Polyhydric alcohols are considered nonionic gradient media. Some of the first centrifugation techniques developed in the 1950s used sucrose in the purification of cell organelles. Sucrose gradients are widely used for the rate-zonal separation of macromolecules and for the isopycnic separation of viruses and cell organelles. The advantages are its stable nature, inertness and low cost. The disadvantages lie in the fact that concentrated solution is viscous and hypertonic. Reagent-grade sucrose may be contaminated with RNAses or heavy metals and therefore are unsuitable for DNA and RNA purification. Glycerol solution is less dense than corresponding sucrose solutions. However, glycerol solution of the same density of sucrose solutions is much more viscous. Glycerol helps to prevent the activity of certain enzymes and it can be removed through vacuum.

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G5516 Glycerol for molecular biology, ≥99.0%
G6279 Glycerol BioXtra, ≥99% (GC)
G9012 Glycerol ≥99.5%
49767 Glycerol BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
240850 D-Sorbitol 99%
S1876 D-Sorbitol ≥98%
85529 D-Sorbitol BioUltra, ≥99.5% (HPLC)
S6021 D-Sorbitol for molecular biology, ≥98%
S7903 Sucrose BioXtra, ≥99.5% (GC)
S9378 Sucrose ≥99.5% (GC)
S0389 Sucrose for molecular biology, ≥99.5% (GC)