BRDU Cell Proliferation Assay Reagents

A broad range of biological and biomedical investigations depend on the ability to distinguish DNA synthesizing cells. Until recently, these studies were often limited by the traditional measurement techniques that are based on the determination of incorporated radioactive DNA precursors such as tritiated thymidine. BromodeoxyUridine (5-Bromo-2-DeoxyUridine, BrdU) is a pyrimidine analogue of thymidine, selectively incorporated into cell DNA at the S phase of the cell cycle. The use of BrdU as a thymidine analogue has made the identification of DNA synthesis in suspensions of cells, cell smears and tissue sections possible. The application of monoclonal antibodies which react specifically with BrdU for detection of DNA replication in lymphoid cells and other normal or pathological preparations, following in vivo or in vitro BrdU labeling, is extensively documented in the biomedical literature. Monoclonal antibodies against BrdU have also proven valuable for studies on cell cycle kinetics, repair synthesis of DNA, demonstration of sister chromatid exchange and assessment of cell proliferation in the presence of growth factors or cytotoxic drugs.
Proliferating cells in the eye of E4 chick embryo from R.S. Goldstein, Dept. of Life Science, Bar-
Hetrokaryons between proliferating (BrdU pre-labeled) and quiescent (unlabeled) endothelial cells

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AEC101 AEC Staining Kit liquid
A9647 Bovine Serum Albumin heat shock fraction, pH 7, ≥98%
B2531 Monoclonal Anti-BrdU antibody produced in mouse clone BU-33, ascites fluid, Immunohistology Grade
B8434 Anti-BrdU antibody, Mouse monoclonal clone BU-33, purified from hybridoma cell culture
B5002 5-Bromo-2′-deoxyuridine ≥99% (HPLC)
B9285 5-Bromo-2′-deoxyuridine BioUltra, ≥99%
D2650 Dimethyl sulfoxide Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
GG1 Glycerol Gelatin aqueous slide mounting medium
G9023 Goat serum
EXTRA2 Mouse ExtrAvidin® Peroxidase Staining Kit antibody produced in goat
P7012 Pepsin from porcine gastric mucosa lyophilized powder, ≥2,500 units/mg protein (E1%/280)
P3563 Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
T7409 Trypsin from porcine pancreas lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid