Kidney Drug Transporter/Toxicity Models

To aid in the investigation of specific drug transporters within the kidney barrier and kidney toxicity, Sigma has generated transporter knockout (KO) cell lines using CompoZr Zinc Finger Nuclease (ZFN) technology in a proprietary Renal Proximal Tubule Epithelial Cells (RPTECs). The RPTEC clone is ideal in that: it expresses multiple transporters, including, OAT1, OAT3, OCT2, MDR1, MRP2, MRP4 and MATE2; is human derived; and demonstrates normal epithelial morphology in culture relative to primary RPTECs. The transporters targeted include: MDR1 (P-gp), OAT1 and OCT2.