High Throughput Drug Screening

Transcreener® assay method can be used across entire families of nucleotide-dependent enzymes. Instead of using separate assays for a wide array of specific reaction products, such as phosphorylated proteins or lipids, a single nucleotide detection assay can be used for all of the enzymes that generate a common nucleotide product.

These assays rely on direct, highly specific detection of nucleotides using antibodies that are able to distinguish between nucleotides on the basis of a single phosphate group. To generate a signal, the Transcreener® assays use a homogenous, competitive immunoassay format in which the antibodies are paired with high-affinity fluorescent tracers. Displacement of the tracer by the nucleotide being detected causes a change in its fluorescence properties.

The assays have fewer reagents and a less complex mechanism than any other nucleotide detection assay. This also means that the protocol is the simplest and most flexible available: run your enzyme reaction, add Transcreener® reagents with stop mix, and read plates. All of the Transcreener® reagents and the assay signals are stable at room temperature for at least 8 hours. This ensures that data quality does not suffer even in large, automated screens, where the detection mix may be stored in a dispensing deck for long periods at room temperature.

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3023 AptaFluor® SAH Methyltransferase Assay
3013 Transcreener® ADP2 FI Assay
3010 Transcreener® ADP2 FP Assay
3011 Transcreener® ADP2 TR-FRET Red Assay
3015 Transcreener® AMP2/GMP2 FP Assay
3020 Transcreener® AMP2/GMP2 TR-FRET Assay
3017 Transcreener® EPIGEN Methyltransferase Assay
3014 Transcreener® GDP FI Assay
3009 Transcreener® GDP FP Assay
3021 Transcreener® GDP TR-FRET Assay
3018 Transcreener® UDP2 FP Assay
3022 Transcreener® UDP2 TR-FRET Assay