Routine Histology Stains
Sigma-Aldrich is a leading supplier of stains, chemicals and kits for use in routine histology. Various formulations of Hematoxylin and Eosin are supplied as ready-to-use solutions in several package sizes. Select from Harris, Gill′s Numbers 1, 2, or 3, Mayer′s Hematoxylin, or alcoholic or aqueous Eosin Y. If you want to prepare your own staining solutions, we offer Hematoxylin and Eosin dyes, and other necessary chemicals and reagents. The routine histology product line includes reagents "For In Vitro Diagnostic Use" for clinical labs.

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B8438 Alcian Blue solution 1% in 3% acetic acid, pH 2.5 Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
    • B8563 Aniline Blue solution 2.5% in 2% acetic acid Aniline blue is used to stain collagen fibers in tissue sections using the Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. It has also been used to stain and visualize glycosylated proteins.
    • HT10132 Bouin’s solution Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.
    • HT101128 Bouin’s solution Bouin′s solution has been used:
    • in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
    • in the fixation of rat ovaries for the observing follicle cells using microscopy
    • in the fixation of gonads of crab for morphological and histological analysis


    Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.
    • 60610 Canada balsam for microscopy Canada balsam is used as a stable apolar hydrophobic mounting medium for light microscopy slide preparation, especially when long term storage is desired.
    • 03984 Canada balsam solution for microscopy embedding medium for microscopy
    • V5265 Crystal violet solution 1%, aqueous solution Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
    Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.
    • D0818 Decalcifying Solution-Lite aqueous solution, bp 208 °F Decalcifying Solution-Lite is designed to be a universal effective decalcifying agent. It is intended to be used for the decalcification of routine, immunohistochemical and bone marrow core specimens. Decalcifying Solution-Lite has been tailored to suit your specific lab routine. It works equally well with all types of specimens in an easy to handle time frame.
    • A3179 Differentiation Solution Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
    • A3429 Differentiation Solution Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
    • HT110116 Eosin Y solution, alcoholic General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110132 Eosin Y solution, alcoholic General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT1101128 Eosin Y solution, alcoholic General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110180 Eosin Y solution, alcoholic General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110332 Eosin Y solution, alcoholic, with phloxine General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110316 Eosin Y solution, alcoholic, with phloxine General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT1103128 Eosin Y solution, alcoholic, with phloxine General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110380 Eosin Y solution, alcoholic, with phloxine General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110216 Eosin Y solution, aqueous General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110232 Eosin Y solution, aqueous General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT110280 Eosin Y solution, aqueous General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • HT1102128 Eosin Y solution, aqueous General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
    • 45348 Epoxy embedding medium, accelerator ≥95% (NT) Epoxy embedding medium, accelerator is used according to the protocol provided for Epoxy Embedding Medium kit, Prod. No. 45359 available on-line. The embedding media is used for electron microscopy.
    • 45359 Epoxy Embedding Medium kit Epoxy Embedding Medium (Epon_812 substitute) is a very widely used embedding resin for electron microscopy, as it penetrates into the tissue specimen faster than Araldite and other polymers due to its low viscosity. Epoxy can be hardened easely and uniformly at low temperatures by the addition of DDSA, NMA and the accelerator DPM-30. Slight shrinkage does occur during curing. Epoxy embedding medium is useful for embedding a variety of tissues as a wide hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and NMA). Universal embedding medium for semi- and ultrathin sections.
    • F5554 Formalin, 10%, Neutral Buffered, Wintergreen Scented Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
    • F5304 Formalin, 10%, Neutral Buffered with 0.03% Eosin Intended for use as general histological fixative. The addition of the eosin to the formulation allows the user to more easily visualize small fragments of tissue during the transfer of the tissue from the fixative to the tissue cassette.
    • HT501850 Formalin solution, neutral buffered, 10% histological tissue fixative Formalin solution, neutral buffered, 10% may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
    Popular general purpose histological fixative.
    • GG1 Glycerol Gelatin aqueous slide mounting medium Glycerol Gelatin has been used for mounting endometrial tissue and pancreatic sections for microscopic examination. It has also been used for the immobilization and mounting of helminth eggs for on chip image analysis.
    • GHS132 Hematoxylin Solution, Gill No. 1 Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.
    • GHS116 Hematoxylin Solution, Gill No. 1 Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.
    • GHS1128 Hematoxylin Solution, Gill No. 1 Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.
    • GHS216 Hematoxylin Solution, Gill No. 2 Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
    • GHS232 Hematoxylin Solution, Gill No. 2 Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
    • GHS280 Hematoxylin Solution, Gill No. 2 Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
    • GHS2128 Hematoxylin Solution, Gill No. 2 Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
    • GHS316 Hematoxylin Solution, Gill No. 3 Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
    • GHS332 Hematoxylin Solution, Gill No. 3 Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
    • GHS3128 Hematoxylin Solution, Gill No. 3 Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
    • GHS380 Hematoxylin Solution, Gill No. 3 Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
    • HHS16 Hematoxylin Solution, Harris Modified General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
    • HHS32 Hematoxylin Solution, Harris Modified General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
    • HHS128 Hematoxylin Solution, Harris Modified General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
    • HHS80 Hematoxylin Solution, Harris Modified General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
    • MHS32 Hematoxylin Solution, Mayer’s General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
    • MHS16 Hematoxylin Solution, Mayer’s General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
    • MHS1 Hematoxylin Solution, Mayer’s General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
    • MHS80 Hematoxylin Solution, Mayer’s General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
    • MHS128 Hematoxylin Solution, Mayer’s General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
    • 56822 Immersion oil for microscopy Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.
    • 62660 LR Accelerator LR Accelerator is used for the chemical curing of LR White and LR Gold embedding media for routine and low temperature electron microscopy or the immunocytochemical localization of antigens by either light or electron microscopy.
    • N3020 Nuclear Fast Red solution Used as a red nuclear counterstain. Nuclear Fast Red solution has been used:
    • In the dual-beam (FIB (focused ion beam)/SEM (scanning electron microscopy)) analysis of intracellular MNPs (magnetic nanoparticles) distribution.
    • As a counterstain in LacZ tissue histological analysis, and in extracellular matrix proteoglycan staining.
    •  In situ hybridization.
    • O1391 Oil Red O solution 0.5% in isopropanol Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
    Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
    • O1516 Oil Red O solution 0.5% in propylene glycol Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
    Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.
    • HT40332 Papanicolaou Stain, EA 50 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40316 Papanicolaou Stain, EA 50 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT403128 Papanicolaou Stain, EA 50 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40380 Papanicolaou Stain, EA 50 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40432 Papanicolaou Stain, EA 65 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40232 Papanicolaou Stain, Modified EA Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40132 Papanicolaou Stain, OG-6 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40180 Papanicolaou Stain, OG-6 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT40116 Papanicolaou Stain, OG-6 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • HT401128 Papanicolaou Stain, OG-6 Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
    • 76258 Paraplast Plus® for electron microscopy, pellets (fast-melting) Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.
    • P8165 Phosphate Buffer pH 6.6 at 25 °C For use with Wright Stain for Geometric Data Hemastainer®.
    • P3288 Phosphate buffer pH 7.2 at 25 °C for hematology and histology staining techniques Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.
    • 02370 Propyl gallate for microscopy, ≥98.0% (HPLC) Propyl gallate is used as an anti-fade reagent in fluorescence microscopy to reduce photobleaching of fluorescent probes.
    Used as an anti-fade reagent in fluorescence microscopy to reduce photobleaching of fluorescent probes such as rhodamine and fluorescein.
    • R3154 Reagent Alcohol 70%, used for histology tissue preparation For use in most histology and cytology procedures.
    • R8382 Reagent Alcohol used for histology tissue preparation Reagent alcohol has been used in most histology and cytology procedures for histology tissue preparation.
    • R3404 Reagent Alcohol 95%, Used for histology tissue preparation For use in most histology and cytology procedures.
    • R3279 Reagent Alcohol 80%, used for histology tissue preparation For use in most histology and cytology procedures.
    • S5134 Scott′s Tap Water Substitute Concentrate 10 × Used as a "blueing reagent" in hematoxylin and eosin staining procedures. Scott′s Tap Water Substitute Concentrate has been used in transferase−mediated dUTP nick end labeling (TUNEL) assay. It has been used in immunohistochemical analysis.
    • HT1079 Weigert’s iron hematoxylin solution Set Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.
    • HT109 Weigert’s iron hematoxylin solution Part B To be used in conjunction with HT107. Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.
    • HT107 Weigert’s iron hematoxylin solution Part A To be used in conjunction with HT109. Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.
    • A5597 Xylene Substitute Green Alternative Non-toxic xylene replacement. Compatible with all tissue processors. May not be compatible with all permanent mounting medias.

    We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of formalin for tissue processing. For more information see www.sigmaaldrich.com/catalog/papers/19608083.