B8438
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Alcian Blue solution 1% in 3% acetic acid, pH 2.5
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Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
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B8563
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Aniline Blue solution 2.5% in 2% acetic acid
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Aniline blue is used to stain collagen fibers in tissue sections using the Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. It has also been used to stain and visualize glycosylated proteins.
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HT10132
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Bouin’s solution
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Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.
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HT101128
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Bouin’s solution
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Bouin′s solution has been used: • in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging • in the fixation of rat ovaries for the observing follicle cells using microscopy • in the fixation of gonads of crab for morphological and histological analysis
Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.
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60610
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Canada balsam for microscopy
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Canada balsam is used as a stable apolar hydrophobic mounting medium for light microscopy slide preparation, especially when long term storage is desired.
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03984
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Canada balsam solution for microscopy
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embedding medium for microscopy
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V5265
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Crystal violet solution 1%, aqueous solution
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Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining. Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.
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D0818
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Decalcifying Solution-Lite aqueous solution, bp 208 °F
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Decalcifying Solution-Lite is designed to be a universal effective decalcifying agent. It is intended to be used for the decalcification of routine, immunohistochemical and bone marrow core specimens. Decalcifying Solution-Lite has been tailored to suit your specific lab routine. It works equally well with all types of specimens in an easy to handle time frame.
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A3179
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Differentiation Solution
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Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
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A3429
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Differentiation Solution
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Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
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HT110116
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Eosin Y solution, alcoholic
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110132
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Eosin Y solution, alcoholic
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT1101128
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Eosin Y solution, alcoholic
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110180
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Eosin Y solution, alcoholic
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110332
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Eosin Y solution, alcoholic, with phloxine
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110316
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Eosin Y solution, alcoholic, with phloxine
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT1103128
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Eosin Y solution, alcoholic, with phloxine
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110380
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Eosin Y solution, alcoholic, with phloxine
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110216
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Eosin Y solution, aqueous
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110232
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Eosin Y solution, aqueous
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT110280
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Eosin Y solution, aqueous
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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HT1102128
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Eosin Y solution, aqueous
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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
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45348
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Epoxy embedding medium, accelerator ≥95% (NT)
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Epoxy embedding medium, accelerator is used according to the protocol provided for Epoxy Embedding Medium kit, Prod. No. 45359 available on-line. The embedding media is used for electron microscopy.
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45359
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Epoxy Embedding Medium kit
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Epoxy Embedding Medium (Epon_812 substitute) is a very widely used embedding resin for electron microscopy, as it penetrates into the tissue specimen faster than Araldite and other polymers due to its low viscosity. Epoxy can be hardened easely and uniformly at low temperatures by the addition of DDSA, NMA and the accelerator DPM-30. Slight shrinkage does occur during curing. Epoxy embedding medium is useful for embedding a variety of tissues as a wide hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and NMA). Universal embedding medium for semi- and ultrathin sections.
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F5554
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Formalin, 10%, Neutral Buffered, Wintergreen Scented
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Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
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F5304
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Formalin, 10%, Neutral Buffered with 0.03% Eosin
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Intended for use as general histological fixative. The addition of the eosin to the formulation allows the user to more easily visualize small fragments of tissue during the transfer of the tissue from the fixative to the tissue cassette.
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HT501850
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Formalin solution, neutral buffered, 10% histological tissue fixative
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Formalin solution, neutral buffered, 10% may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology. Popular general purpose histological fixative.
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GG1
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Glycerol Gelatin aqueous slide mounting medium
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Glycerol Gelatin has been used for mounting endometrial tissue and pancreatic sections for microscopic examination. It has also been used for the immobilization and mounting of helminth eggs for on chip image analysis.
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GHS132
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Hematoxylin Solution, Gill No. 1
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Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.
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GHS116
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Hematoxylin Solution, Gill No. 1
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Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.
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GHS1128
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Hematoxylin Solution, Gill No. 1
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Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.
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GHS216
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Hematoxylin Solution, Gill No. 2
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Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
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GHS232
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Hematoxylin Solution, Gill No. 2
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Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
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GHS280
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Hematoxylin Solution, Gill No. 2
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Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
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GHS2128
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Hematoxylin Solution, Gill No. 2
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Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.
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GHS316
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Hematoxylin Solution, Gill No. 3
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Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
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GHS332
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Hematoxylin Solution, Gill No. 3
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Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
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GHS3128
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Hematoxylin Solution, Gill No. 3
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Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
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GHS380
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Hematoxylin Solution, Gill No. 3
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Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.. Used with hematoxylin and eosin staining.
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HHS16
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Hematoxylin Solution, Harris Modified
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General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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HHS32
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Hematoxylin Solution, Harris Modified
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General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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HHS128
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Hematoxylin Solution, Harris Modified
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General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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HHS80
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Hematoxylin Solution, Harris Modified
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General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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MHS32
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Hematoxylin Solution, Mayer’s
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General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
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MHS16
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Hematoxylin Solution, Mayer’s
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General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
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MHS1
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Hematoxylin Solution, Mayer’s
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General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
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MHS80
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Hematoxylin Solution, Mayer’s
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General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
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MHS128
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Hematoxylin Solution, Mayer’s
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General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
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56822
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Immersion oil for microscopy
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Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.
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62660
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LR Accelerator
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LR Accelerator is used for the chemical curing of LR White and LR Gold embedding media for routine and low temperature electron microscopy or the immunocytochemical localization of antigens by either light or electron microscopy.
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N3020
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Nuclear Fast Red solution
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Used as a red nuclear counterstain. Nuclear Fast Red solution has been used: • In the dual-beam (FIB (focused ion beam)/SEM (scanning electron microscopy)) analysis of intracellular MNPs (magnetic nanoparticles) distribution. • As a counterstain in LacZ tissue histological analysis, and in extracellular matrix proteoglycan staining. • In situ hybridization.
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O1391
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Oil Red O solution 0.5% in isopropanol
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Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue. Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
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O1516
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Oil Red O solution 0.5% in propylene glycol
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Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue. Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.
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HT40332
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Papanicolaou Stain, EA 50
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40316
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Papanicolaou Stain, EA 50
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT403128
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Papanicolaou Stain, EA 50
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40380
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Papanicolaou Stain, EA 50
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40432
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Papanicolaou Stain, EA 65
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40232
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Papanicolaou Stain, Modified EA
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40132
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Papanicolaou Stain, OG-6
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40180
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Papanicolaou Stain, OG-6
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT40116
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Papanicolaou Stain, OG-6
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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HT401128
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Papanicolaou Stain, OG-6
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Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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76258
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Paraplast Plus® for electron microscopy, pellets (fast-melting)
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Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.
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P8165
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Phosphate Buffer pH 6.6 at 25 °C
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For use with Wright Stain for Geometric Data Hemastainer®.
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P3288
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Phosphate buffer pH 7.2 at 25 °C for hematology and histology staining techniques
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Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.
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02370
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Propyl gallate for microscopy, ≥98.0% (HPLC)
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Propyl gallate is used as an anti-fade reagent in fluorescence microscopy to reduce photobleaching of fluorescent probes. Used as an anti-fade reagent in fluorescence microscopy to reduce photobleaching of fluorescent probes such as rhodamine and fluorescein.
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R3154
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Reagent Alcohol 70%, used for histology tissue preparation
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For use in most histology and cytology procedures.
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R8382
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Reagent Alcohol used for histology tissue preparation
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Reagent alcohol has been used in most histology and cytology procedures for histology tissue preparation.
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R3404
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Reagent Alcohol 95%, Used for histology tissue preparation
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For use in most histology and cytology procedures.
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R3279
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Reagent Alcohol 80%, used for histology tissue preparation
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For use in most histology and cytology procedures.
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S5134
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Scott′s Tap Water Substitute Concentrate 10 ×
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Used as a "blueing reagent" in hematoxylin and eosin staining procedures. Scott′s Tap Water Substitute Concentrate has been used in transferase−mediated dUTP nick end labeling (TUNEL) assay. It has been used in immunohistochemical analysis.
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HT1079
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Weigert’s iron hematoxylin solution Set
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Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.
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HT109
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Weigert’s iron hematoxylin solution Part B
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To be used in conjunction with HT107. Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.
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HT107
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Weigert’s iron hematoxylin solution Part A
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To be used in conjunction with HT109. Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.
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A5597
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Xylene Substitute
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Non-toxic xylene replacement. Compatible with all tissue processors. May not be compatible with all permanent mounting medias.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of formalin for tissue processing. For more information see www.sigmaaldrich.com/catalog/papers/19608083.
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