Fluorescent Dye Wavelength Index

Fluorescent dyes are used to tag proteins, nucleic acids, and other biomolecules for use in life science applications, including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, and enzyme assays. Using the right combination of fluorescent-labeled biomolecules can enable multiple targets to be visualized simultaneously. Fluorescent dyes are optimized for excitation and detection in the visible light to near infrared spectrum. The choice of fluorochrome to use is determined by its excitation and emission wavelengths and the application.

To sort products within a column in the table below, click on the column heading. To re-alphabetize columns, refresh your web browser.

Cat. No. Product Name Solvent λ Ex Max λ Em Max Additional Conditions for Fluorescence λ Ex Max λ Em Max
01673 Acriflavine EtOH 463 490      
06627 2-Aminoacridone pH 8.0 420 538      
08445 7-Amino-4-methyl-3-coumarinylacetic acid MeOH 350 433      
08658 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt pH 7.0 356 512      
09341 8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt pH 7.4 420 500      
25102 9-(Chloromethyl)anthracene       Carboxylic acid adducts 365 412
10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt MeOH 372 480 Protein adduct 372 480
10580 Anthracene CH3CN 251 401      
10609 9-Anthracenecarbonyl cyanide MeOH 361 460 Hydroxyl compound adduct with ethanol 361 451
75068 9,10-Anthracenediyl-bis(methylene)dimalonic acid pH 7.0 380 407      
89313 Atto 390   390  475       
89740 Atto 390 maleimide   390  472       
89204 Atto 390 NHS ester   389  471       
16805 Atto 425-NHS ester   437  483       
50712 Atto 465            
55607 Atto 465 maleimide   452  502       
53404 Atto 465-NHS ester   449  503       
41051 Atto 488   504  521       
28562 Atto 488 maleimide   501  523       
41698 Atto 488-NHS ester   498  520       
16951 Atto 495   499  535       
41022 Atto 495 maleimide   500  524       
00379 Atto 495-NHS ester   499  524       
16590 Atto 520-maleimide            
77810 Atto 520 NHS ester H2O 520 542 EtOH 525 547
06699 Atto 532   533  560       
68499 Atto 532 maleinimide   532  553       
88793 Atto 532 NHS ester   530  551       
40592 Atto 540 Q            
62453 Atto 540 Q maleimide            
61683 Atto 540 Q NHS ester            
42424 Atto 550   554  574       
30730 Atto 550 maleimide   554  576       
92835 Atto 550 NHS ester   539  575       
75784 Atto 565 H2O 561 585 EtOH 566 590
72464 Atto 565 NHS ester H2O 561 585 EtOH 566 590
03722 Atto 580 Q            
68152 Atto 580 Q maleimide            
70425 Atto 590 H2O 598 634 EtOH 598 634
79636 Atto 590 NHS ester H2O 598 634 EtOH 598 634
08637 Atto 594   601  627       
08717 Atto 594 maleimide   601  627       
08741 Atto 594 NHS ester   597  625       
78493 Atto 610 H2O 605 646 EtOH 616 630
78493 Atto 610   610  635       
41061 Atto 610-maleimide   610  637       
93259 Atto 610 N-succinimidyl ester H2O 605 646 EtOH 616 630
93259 Atto 610 N-succinimidyl ester   610  635       
04327 Atto 612 Q            
50332 Atto 612 Q maleimide            
53988 Atto 612 Q NHS ester            
92716 Atto 620   617  641       
67351 Atto 620 NHS ester   620  641       
18620 Atto 633            
01464 Atto 633 NHS ester   630  650       
08968 Atto 635   635  656       
43429 Atto 635 NHS ester   624  655       
97875 Atto 647   643  665       
41784 Atto 647 maleimide   647  669       
04507 Atto 647N   644  667       
07376 Atto 647 NHS ester   643  662       
05316 Atto 647N maleimide   644  669       
18373 Atto 647N NHS ester   647  661       
93711 Atto 655 H2O 665 690 EtOH 655 680
76245 Atto 655 NHS ester H2O 665 690 EtOH 655 680
94875 Atto 680 H2O 680 702 EtOH 675 699
75999 Atto 680 NHS ester H2O 680 702 EtOH 675 699
30674 Atto 700   681  714       
50611 Atto 700 maleimide   645  715       
16986 Atto 700 NHS-ester   681  713       
47156 Atto 725   725  752       
93725 Atto 725 NHS ester   725  753       
91394 Atto 740   740  764       
14448 2-(4-Biphenylyl)-6-phenylbenzoxazole H2O   425 CH3CN, MeOH   390
14510 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid pH 8.0 287 363 with calcium 274 363
14513 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrasodium salt pH 8.0 254 363 with calcium 274 363
14530 bisBenzimide H 33258 pH 7.0 338 505 bound to DNA 356 465
14533 bisBenzimide H 33342 trihydrochloride pH 7.0 340 510 bound to DNA 355 465
14562 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester       Reaction product 482 528
15025 meso-1,2-Bis(4-methoxyphenyl)ethylenediamine       Saccharide adducts 340 460
15090 1,4-Bis(2-methylstyryl)benzene Cyclohexane   419 Heptane   416
69898 Bromobimane pH 7.0 398   Thiol adduct 390 478
72301 9-(2-Carboxy-2-cyanovinyl)julolidine pH 8.0 433 500      
21882 5(6)-Carboxy-2',7'-dichlorofluorescein pH 8.0 504 529      
21884 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate       Reaction product 470 504
86826 5-Carboxyfluorescein pH 8.0 492 517      
21877 5(6)-Carboxyfluorescein pH > 8.0 490 520      
21879 5(6)-Carboxyfluorescein diacetate       hydrolyzed 492 517
21888 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester DMF <300   hydrolyzed 492 517
21878 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester pH 9.0 495 519      
92846 5-Carboxyfluorescein N-succinimidyl ester pH 8.0 492 517      
21965 5(6)-Carboxy-X-rhodamine MeOH 568 595      
41969 5-Carboxy-X-rhodamine N-succinimidyl ester pH 7.0 575 600      
21953 5(6)-Carboxytetramethylrhodamine MeOH 541 566      
55222 6-Carboxytetramethylrhodamine pH 8.0 543 575      
53048 5-Carboxy-tetramethylrhodamine N-succinimidyl ester pH 7.0 543 575      
21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester MeOH 540 565      
88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester pH 7.0 543 575      
25455 4-Chloro-7-nitrobenzofurazan MeOH 336   Aliphatic amine adducts 475 540
30423 3-(Dansylamino)phenylboronic acid MeOH 337 517 Protein adduct 337 490
30432 Dansylcadaverine MeOH 335 518 Reaction product 338 500
39220 Dansyl chloride CHCl3 372   Butylamine adduct 337 492
30434 Dansylhydrazine MeOH 336 534 Saccharide adducts 336 487
32670 4',6-Diamidino-2-phenylindole dihydrochloride H2O     bound to DNA 358 461
34025 Dibromobimane MeOH 391   Thiol adduct 391 480
72335 9-(2,2-Dicyanovinyl)julolidine pH 8.0 433 500      
36798 7-(Diethylamino)coumarin-3-carbohydrazide MeOH 420 468 Carboxylic acid adduct 415 480
31755 7-(Diethylamino)coumarin-3-carbonyl azide MeOH 432 480      
36799 7-(Diethylamino)coumarin-3-carboxylic acid pH 9.0 409 473      
36801 7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester MeOH 432 472      
37150 3,3'-Diheptylthiacarbocyanine iodide MeOH 562 575      
37291 Dihydroethidium MeCN 355 420 bound to DNA 358 461
37920 6,8-Dihydroxy-1,3-pyrenedisulfonic acid disodium salt pH 4.0 405 465 pH 8.0 458 500
39235 11-[5-(Dimethylamino)-1-naphthalenesulfonylamino]undecanoic acid MeOH 335 519      
39255 4-Dimethylamino-4'-nitrostilbene CH2Cl2 500 700      
41525 N,N-Dimethyl-6-propionyl-2-naphthylamine MeOH 361 498      
41785 Dimidium bromide pH 7.6, low ionic strength 480   Nucleic acid adducts, Tris pH 8.0 306 612
42364 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate MeOH 550 565      
43608 3,3'-Dipropylthiadicarbocyanine iodide MeOH 651 675      
40227 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine MeOH 364 497      
44583 Doxorubicin hydrochloride EtOH 470 585      
45245 Eosin 5-isothiocyanate pH 9.0 521 544      
46067 Ethidium bromide solution H2O 480 620 bound to DNA 518 605
46043 Ethidium homodimer H2O 493   bound to DNA 528 617
46123 1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide H2O 355 460      
46125 8-Ethoxy-N,N,N',N',N'',N''-hexamethylpyrene-1,3,6-trisulfonamide       Reaction product 380 520
46393 Fluo-3 pH > 6.0 503   with calcium 506 526
46917 9-Fluorenylmethyl carbazate MeOH 264 309 Saccharide adducts 270 320
46955 Fluorescein pH 9.0 490 514      
46960 Fluorescein sodium salt pH 9.0 490 514      
46935 6-[Fluorescein-5(6)-carboxamido]hexanoic acid pH 9.0 480 525      
46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester pH 8.2 491 515      
46943 Fluorescein dilaurate       Reaction product 490 514
46950 Fluorescein 5(6)-isothiocyanate pH 9.0 495 520      
46946 Fluorescein isothiocyanate–dextran 150000 pH 8.0 490 520      
46945 Fluorescein isothiocyanate–dextran 70000 pH 8.0 490 520      
46947 Fluorescein isothiocyanate – Dextran 500000-Conjugate pH 8.0 490 520      
46985 Fluorescein-5-thiosemicarbazide pH 9.0 492 516      
55817 Fluorescent red 630 pH 7.0 620 650      
47140 4-Fluoro-7-nitrobenzofurazan EtOH 470 528 Amino acid adducts 470 530
47614 Fluram® pH 9.0 234   Protein adduct 334 455
52335 N-Hexadecylpyrene-1-sulfonamide MeOH 350 400      
55155 7-Hydroxycoumarin-3-carboxylic acid pH 4.0 342 447 pH 9.0 386 448
55156 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester MeOH 418 447      
55634 7-Hydroxy-4-methyl-3-coumarinylacetic acid pH 10.0 360 445      
55627 7-Hydroxy-4-methyl-2(1H)-quinolone pH 5.0 321 357 pH 9.0 351 438
56059 7-Hydroxy-N-octadecylcoumarin-3-carboxamide CHCl3/DMSO 359 406      
57180 Indo 1 100 mM KCl 349 482 with calcium 331 398
64951 7-Methoxycoumarin CHCl3 340 389 Reaction product 360 449
64948 7-Methoxycoumarin-3-carboxylic acid MeOH 360 415      
64958 2-Methoxy-2,4-diphenyl-3(2H)-furanone       Amine adducts 390 480
69025 1-Methylpyrene CHCl3 340 486      
69587 2'-(4-Methylumbelliferyl)-a- D -N-acetylneuraminic acid sodium salt hydrate pH 7.0 315 374 Reaction product 360 449
69585 4-Methylumbelliferyl N-acetyl-ß- D -glucosaminide dihydrate pH 10.0 317   Reaction product 360 449
19362 4-Methylumbelliferyl butyrate MeOH 312   Reaction product 360 449
69591 4-Methylumbelliferyl a- D -glucopyranoside pH 9.0 317 374 Reaction product 360 449
69602 4-Methylumbelliferyl-ß- D -glucuronide hydrate pH 9.0 316 375 Reaction product 360 449
51010 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate pH 6.0 323 464 Reaction product 360 449
75164 4-Methylumbelliferyl oleate MeOH 312   Reaction product 360 449
69899 Monochlorobimane MeOH 380 461 Thiol adduct 380 480
70420 Naphthofluorescein pH 10.0 594 663      
70450 2-Naphthol 0.1 M NaOH 320 410      
70448 2-Naphthol 0.1 M NaOH 320 410      
70684 1-(2-Naphthoyl)imidazole H2O 230 362 Aldite adducts 234 374
15167 NIR-797 isothiocyanate MeOH 668 798      
72963 12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid MeOH 467 539      
72964 6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid MeOH 467 539      
74758 8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt H2O 404 434      
74875 8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt H2O 384 409 Reaction product 460 520
75926 Oxonol VI MeOH 599 634      
76275 PBFI-AM MeOH 369   with Na 334 525
77490 Phenanthridine DMSO 265 415      
79279 3-Phenylumbelliferone pH 3.0 338 463 pH 10.0 383 472
79760 Phthaldialdehyde pH 7.0 257   Thiol adduct 334 455
81845 Propidium iodide pH 7.2 482 608 bound to DNA 540 608
82648 Pyrene n-Pentane 350 392      
82660 1-Pyrenedecanoic acid MeOH 341 377      
82663 1-Pyrenedodecanoic acid MeOH 341 377      
82657 1-Pyrenesulfonic acid sodium salt MeOH 344 376      
08520 Quin-2       with calcium 332 493
22620 Quinine 0.5 M Sulfuric acid 347 448      
22640 Quinine hemisulfate salt monohydrate H2O 343 453      
22630 Quinine hydrochloride dihydrate H2O 322 450      
83636 Resorufin acetate DMSO 540 590 Reaction product 571 585
83637 Resorufin butyrate DMSO 540 590 Reaction product 571 585
46121 Resorufin ethyl ether MeOH 464        
69125 Resorufin methyl ether MeOH 463        
83689 Rhodamine B Acidic EtOH 554 627      
83697 Rhodamine 6G MeOH 528 551      
83694 Rhodamine 101 MeOH 565 589      
83695 Rhodamine 110 chloride MeOH 499 521      
83701 Rhodamine 800 MeOH 528 547      
83685 Rhodamine B octadecyl ester perchlorate MeOH 554 578      
17866 Streptavidin-CryptoFluor™ Crimson pH 7.2 585 658      
86186 Sulforhodamine B acid chloride H2O 565 586      
86470 p-Terphenyl EtOH 277        
87917 Tetramethylrhodamine ethyl ester perchlorate MeOH 549 574      
87918 Tetramethylrhodamine isothiocyanate mixed isomers MeOH 543 571      
88071 meso-Tetraphenylporphyrin Toluene 645 651      
43060 N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate MeOH 355 450      
93979 Umbelliferone 95% EtOH 325 441