DMEM/Nutrient Mixture F-12 Ham (DMEM/F12)

DMEM:F12 is a 50:50 mixture of DMEM and Ham’s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Product #

Description

Type

Glucose

L-Glutamine

Phenol Red

Pyruvate

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D9785 DMEM/Nutrient Mixture F-12 Ham With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture 3.15 no no 0.055
51445C Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 liquid, suitable for cell culture 3.15 yes yes 0.055
51448C Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 suitable for cell culture 3.15 yes yes 0.055
56498C Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 powder, with 3151 mg/L dextrose, with 2.5 mM L-glutamine, with 55 mg/L sodium pyruvate, without sodium bicarbonate 3.15 yes yes 0.055
D8437 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture 3.15 yes yes 0.055
D6421 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture 3.15 no yes 0.055
D6434 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture 3.15 no no 0.055
D8062 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture 3.15 yes yes 0.055
D8900 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture 3.15 yes yes 0.055
D2906 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture 3.15 yes no 0.055
D0547 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture 3.15 yes yes 0.11