Stable Glutamine Media and Supplements

There are several amino acids which are necessary for most cell culture systems. Among these, L-glutamine is one such essential component that has been shown to degrade in liquid medium over time under typical growth conditions. Click here for more information.

Maintaining required levels of L-glutamine in cell culture often requires the repeated supplementation of the medium with additional L-glutamine, which can lead to the build of toxic levels of ammonia over time, as well as pose as a risk of potential contamination. Therefore, the stability of L-glutamine has become a key concern in research and industry when seeking to increase culture longevity and density while preventing the compromise of viability and growth.

Alanyl-glutamine (Ala-Gln), also known as stable glutamine, is a dipeptide that is can be used as a substitute for L-glutamine in many mammalian cell culture systems. Stable glutamine is much less labile in solution, is stable to heat sterilization, and is less ammoniagenic than L-glutamine.

Because of these benefits, we now offer a complete line of stable glutamine media, which are our most popular classical media formulations supplemented with stable glutamine instead of L-glutamine.

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D0697 StableCell DMEM/F12 With stable glutamine, 15mM HEPES and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
D0819 StableCell DMEM - high glucose With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture
D0822 StableCell DMEM - high glucose With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
N3790 StableCell Ham′s F-12 With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
I2911 StableCell IMDM With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M0446 StableCell MEM With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M6199 StableCell MEM, Alpha Modification With stable glutamine and sodium bicarbonate, without ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
M6074 StableCell MEM, Alpha Modification With stable glutamine and sodium bicarbonate, with ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
R2405 StableCell RPMI-1640 With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture