Classical Media and Salts

DMEM (Dulbecco's Modified Eagle's medium)

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM). DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for cultivation of certain cell types.

Product No. Product Description Application
D5546 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 1000 mg/L glucose and sodium bicarbonate
• Without L-glutamine
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered 
Liquid form of the original Dulbecco's formulation, offered as D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D6046 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 1000 mg/L glucose, L-glutamine and
  sodium bicarbonate
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Fully supplemented liquid form of the original Dulbecco's formulation, offered as D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D2429 Dulbecco's Modified
Eagle's Medium (10X)
• Liquid
• With 1000 mg/L glucose at 1x
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Without L-glutamine, supplement with 0.584 gm/L
  L-glutamine, at 1x
• Without sodium bicarbonate, supplement with
  3.7 gm/L sodium bicarbonate, at 1x
• Without folic acid, supplement with 0.004 gm/L
  folic acid, at 1x
• pH 1.8-2.0 at 10x
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Concentrated liquid version of the original low glucose Dulbecco's formulation, with the pyridoxine substitution (see D5546).

Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.
D5523 Dulbecco's Modified
Eagle's Medium
• Powder
• With L-glutamine and 1000 mg/L glucose
• Without sodium bicarbonate, supplement with
  3.7 gm/L  sodium bicarbonate
• Formulated at 10.0 grams of powder per liter of
  medium
• Cell culture tested
Provides investigator with the option to modify the buffering system. Also, this is Dulbecco's original low glucose formulation.
D5921 Dulbecco's Modified
Eagle's Medium Modified
• Liquid
• With 1000 mg/L glucose, and sodium bicarbonate
• Without phenol red and L-glutamine, supplement
  with 0.584 gm/L L-glutamine
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Liquid form of the original Dulbecco's formulation, offered as D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.

See D2902 for note on phenol red
D2902 Dulbecco's Modified
Eagle's Medium Modified
• Powder
• With L-glutamine and 1000 mg/L glucose
• Without sodium bicarbonate, supplement with
  3.7 gm/L of sodium bicarbonate
• Without phenol red
• Formulated at 10.0 grams of powder
  per liter of medium
• Cell culture tested
Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
D5671 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 4500 mg/L glucose and sodium bicarbonate
• Without L-glutamine, supplement with 0.584 gm/L
  L-glutamine
• Cell culture tested
• Hybridoma tested
• Endotoxin tested
• Sterile-filtered
Complete liquid form of the high glucose modification of Dulbecco's original formulation.

Fully tested.
D5796 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 4500 mg/L glucose, L-glutamine and
  sodium bicarbonate
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Complete liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D5648 Dulbecco's Modified
Eagle's Medium
• Powder
• With L-glutamine and 4500 mg/L glucose
• Without sodium bicarbonate, supplement with 
  3.7 gm/L of  sodium bicarbonate
• Formulated at 13.4 grams of powder per liter
  of medium
• Cell culture tested
• Related to DME, Hybridoma tested (D 6655)
Modified version of D5523. Many cell lines have been found to require a higher glucose level than was provided in the original formulation.
D6546 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 4500 mg/L glucose, 110 mg/L sodium pyruvate
  and sodium bicarbonate
• Without L-glutamine, supplement with 0.584 gm/L
  L-glutamine
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.

Glutamine to be added by the investigator
D6429 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 4500 mg/L glucose, L-glutamine, sodium
  pyruvate, and sodium bicarbonate
• With pyridoxine (substitutes pyridoxine HCl for
  pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D7777 Dulbecco's Modified
Eagle's Medium
• Powder
• With L-glutamine,  4500 mg/L glucose and
  110 mg/L sodium pyruvate
• Without sodium bicarbonate, supplement with
  3.7 gm/L of sodium bicarbonate
• Formulated at 13.5 grams of powder
  per liter of medium
• Cell culture tested
• Cell culture tested
Same as D5648, except that it is supplemented with  the additional energy source pyruvate.
D6171 Dulbecco's Modified
Eagle's Medium
HEPES Modified
• Liquid
• With 4500 mg/L glucose, 25 mM
  HEPES and sodium bicarbonate
• Without L-glutamine, supplement with
  0.584 gm/L L-glutamine
• With pyridoxine (substitutes pyridoxine
  HCl for pyridoxal HCl)
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.

See D1152 for discussion of HEPES.
D1152 Dulbecco's Modified
Eagle's Medium
HEPES Modification
• Powder
• With L-glutamine and 4500 mg/L glucose
  and 25 mM HEPES
• Without sodium bicarbonate, supplement with
  3.7 gm/L of sodium bicarbonate
• Formulated at 17.4 grams of powder
  per liter of medium
• Cell culture tested
Same as D5648, except that it is supplemented with HEPES. HEPES supplementation increases the buffering capacity of the medium. Recommended for cells that require a higher pH (7.3-7.6) for optimal growth.
D0422 Dulbecco's Modified
Eagle's Medium
• Liquid
• With 4500 mg/L glucose, and sodium bicarbonate
• Without L-methionine, L-cystine and L-glutamine,
  supplement with  0.584 gm/L  L-glutamine
• Cell culture tested
• Endotoxin tested
• Sterile-filtered
Deficient liquid form of the high glucose version of Dulbecco's original formulation. Key amino acids have been left out to allow researchers to set up metabolic studies with tagged amino acids.
D5030 Dulbecco's Modified
Eagle's Medium Base
• Powder
• Without L-glutamine, glucose, phenol red, sodium
  pyruvate and sodium bicarbonate
• Supplement with 0.584 gm/L of L-glutamine;
  1.0 gm/L glucose; 3.7 gm/L of sodium
  bicarbonate
• Formulated at 8.3 grams of powder
  per liter of medium
• Cell culture tested
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.