Cell Culture

Fundamental Techniques in Cell Culture

 ECACC Laboratory Handbook 4th Edition
 

 Cell Culture Protocols

The European Collection of Authenticated Cell Cultures (ECACC)

The European Collection of Authenticated Cell Cultures (ECACC) was established in 1985 as a cell culture collection to service the research community and provide an International Depository Authority recognized patent depository for Europe. Over the last three decades, ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business. The collection currently holds cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders. ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National Collection of Pathogenic Viruses (NCPV) and the National Collection of Pathogenic Fungi (NCPF).

The development and maintenance of such a diverse collection has inevitably produced a high level of specialist knowledge and this has enabled ECACC to position itself as a center of expertise in all aspects of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into new product areas such as high quality genomic DNA extracted from the cell lines.

Over ten years ago, the European Collection of Authenticated Cell Cultures (ECACC) and our company formed a working partnership to bring together the most diverse selection of cell culture products and services available commercially. We did this with researchers like you in mind, to ensure that you have the quality products you need to advance your research goals. We continue to expand upon this partnership, and now are able to offer an even greater array of cell lines, cell culture products, knowledge and services to the global research community.

To that aim, we have assembled this updated laboratory handbook of cell culture techniques. For the researcher new to cell culture, this handbook provides a wealth of information–from the sourcing of cell lines, laboratory design and safety, to aspects of cryopreservation, quality control and cell line authentication. The issue of cell line misidentification and cross contamination has been recognized as a significant problem in recent years, and solutions are addressed here. Additionally, a series of detailed protocols are provided, which are routinely used in the ECACC laboratories. Protocols for the use of induced pluripotent stem cells and for growing cells in 3D cell culture, two areas of growing importance to cell culture research, have been added to this latest edition of the handbook. The handbook is intended as a guide rather than an in-depth text of cell culture; you are encouraged to consult relevant specialized literature to obtain more detailed information.