Cell Culture

MegaCell Media

Discover and save with our MegaCell™ line of enriched cell culture media products designed to reduce fetal bovine serum (FBS) supplementation to 3% or less.

We have optimized this classic media through fortification of a multitude of specially engineered supplements, growth factors and amino acids.
 

The result? The media you have always wanted: greater growth and performance while saving time and money.

 

MegaCell™ Formulation:
The MegaCell™ line of products is based on the classic basal medium formulations, which may be found in the our Life Science Research catalog. MegaCell™ has been enriched with growth factors, amino acids, insulin, trace elements and non-essential amino acids. In addition, HEPES has been added to every bottle to increase buffering capacity. Serum supplementation was optimized at 3% for a broad range of cellular applications. However, MegaCell™ supplemented with 1-5% FBS performed very well.

View the complete MegaCell™ Brochure! (190 KB PDF)


M3942 MegaCell™ DME:
 

 

MegaCell™ DME supplemented with 4 mM L-Glutamine and 3% FBS (Fetal Bovine Serum) was compared to DME supplemented with 4 mM L-Glutamine and 10% FBS. MDBK (Bovine Kidney) cells were plated without pre-adaptation to MegaCell™ DME at 5000 viable cells/cm2. The flasks were incubated at 37 °C with 5% CO2 and 95% air over a 4-day passage cycle. Cell growth and morphology were superior with MegaCell™.

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M3817 MegaCell™ RPMI-1640:

 

M3817 MegaCell™ RPMI-1640 vs. Opti-MEM®:

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MegaCell™ = Mega Options:

MegaCell™ gives you more options for serum supplementation. Without comprising cell growth or promotion, MegaCell™ supplemented with 6% Newborn Calf Serum or 10% Bovine Serum worked as well as the classic medium + 10% FBS.

 

 

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Common Applications for MegaCell™ + 3% Fetal Bovine Serum

MegaCell™ shows outstanding results on a wide variety of cell lines, including attached and suspension cells.
 

Cell Line Seeding
Density
DME
M3942
RPMI
M3817
VERO (attached) 5000/cm2 - -
MDBK (attached) 5000/cm2 ++++ -
MRC-5 (attached) 5000/cm2 ++ -
WI-38 (attached) 5000/cm2 +++ -
SP20 (suspension) 75000/ml - ++++
HFN (suspension) 75000/ml - ++++

Note: Number of "+" indicates strength of application data results.
Note: MegaCell™ is not recommended for 3T3 or MDCK cells.

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Product Name Product # 
MegaCell™ Dulbecco's Modified Eagle's Medium M3942
MegaCell™ RPMI-1640 Medium M3817


We use the MEGACELL trademark pursuant to an Agreement with Cortex Biochem, Inc.