Cell Culture

Stem Cell Media


 Stemline Platform—Media for expansion and maturation of stem cells

In recent years, the development of media used to expand and mature adult stem cells has greatly increased the success rate of adult stem cell therapy and the breadth of potential stem cell-based therapies. In order to obtain sufficient numbers of cells for applications of this therapeutic approach, ex vivo expansion has been utilized to ensure successful engraftment and economize the manufacture of both allogeneic and autologous therapies. To this end, we have developed the Stemline Platform of cGMP-manufactured media, optimized for the expansion and maturation of various adult stem cell types in clinical and manufacturing environments.

Table 1 - Stemline media product attributes

Product Cell Type GMP manufactured? Serum free? Xeno free? Performance validated: every batch tested for cell expansion Consistency validated: every batch tested for quality
14371C / 14372C Mesenchymal stem cells
S0192 Hematopoietic stem cells (CD34+ and 4-HC-purged CD34+)
S3194 Neural stem cells


 Proven performance

The Stemline media products are designed for maximum cell expansion performance and compatibility with clinically relevant processes.

Tested with MSCs derived from bone marrow, adipose, and other tissues, Stemline XF MSC medium is a xeno-free formulation that offers supply chain security and regulatory friendliness to your clinical process. Unlike other xeno-free solutions which vary significantly in batch-to-batch performance, Stemline XF MSC Medium supports superior, consistent expansion performance of MSCs. Furthermore, Stemline XF MSC Medium has been designed for use in both planar and microcarrier culture formats, and outperforms both αMEM/hPL and a top xeno-free competitor product when used for bone marrow MSC expansion on microcarriers in bench-scale bioreactors.


Figure 1. bmMSCs show improved growth in Stemline XF MSC Medium in the Mobius® 3 L single-use bioreactor relative to αMEM/hPL or a serum-free competitor product (A) while maintaining hMSC surface marker expression (B).


Similarly, Stemline II HSC Expansion Medium promoted significantly more growth than other serum-free medium competitors when expanding bone marrow-derived HSCs. Stemline II HSC expansion medium also supports significantly more growth of umbilical-derived HSCs as its competitors.

Figure 2. Stemline® XF MSC Medium has high yield and functionality in the 3 L Mobius® stirred tank bioreactor. This product was designed for efficient expansion in planar and microcarrier-based culture platforms, easing the transfer between research, clinical, and manufacturing scale culture.


To learn more about Stemline XF MSC Medium, which promotes expansion of human mesenchymal stromal/stem cells (hMSCs) to high densities while maintaining cell identity and quality, view the below technical note.

Stemline® XF MSC Medium has High Yield and Functionality in the 3 L Mobius® Stirred Tank Bioreactor  

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