Biological Buffers


Synonym: N-(2-Acetamido)iminodiacetic acid

ADA, or N-(2-Acetamido)iminodiacetic acid, can be used to study biological buffers and zwitterionic compounds1. This Good's buffer prevents oxidation and irreversible denaturation of proteins while conducting gel electrophoresis2.
Useful pH Range 6.0 - 7.2
Working concentration range 100mM5


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
ADA 26239-55-4 C6H10N2O5 190.15 A9883 00307    
ADA sodium salt 41689-31-0 C6H8N2Na2O5 234.12 A0153      


Interaction Comments

ADA has been shown to interfere with color development in the bicinchoninic acid assay for protein quantitation3.
Strong interaction with Mn, Co, Ni, Cu, Zn, Cd and Pb4


Literature References

  1. Robinson J.D. and Davis R.L. Biochim Biophys Acta. 1987 Apr 30;912(3):343-7.
  2. Righetti P.G., Chiari M, Gelfi C. Electrophoresis. 1988 Feb;9(2):65-73.
  3. Kaushal, V., and Barnes, L. D. Anal. Biochem., 157(2), 291-294 (1986).
  4. Ferreira C.M., Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003.
  5. Talreja S., Elucidation of protein-precipitant phase diagrams and their link to crystal quality, Dissertation Abstracts International, 69-05B. University of Illinois at Urbana-Champaign 2008.