Biological Buffers

BIS-TRIS

BIS-TRIS is an amine buffer very similar to Trizma ("Tris") in structure. BIS-TRIS is an important buffer for protein and nucleic acid systems. It is also used as a substitute for cacodylic acid buffer systems1.
Useful pH Range 5.8 - 7.2
Working concentration range 10mM-50mM5-7

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
BIS-TRIS 6976-37-0 C8H19NO5 209.24 RDD013 B9754 B7535 14879
B4429
RES1161B-A7 PHG0004  
BIS-TRIS hydrochloride 124763-51-5 C8H19NO5 • HCl 245.70 B6032   RES1164B-A7  

 

Interaction Comments
 

BIS-TRIS binds Cu and Pb. Weakly binds Mg, Ca, Mn, Co, Ni, Zn and Cd4

 

Literature References

  1. Scopes, R.K. ed. (1994) Protein Purification (Principles and Practice) 3rd Edition, p. 352; Springer-Verlag.
  2. Sheller, Kurt H., et al. European Journal of Biochemistry. 107, 455-466 (1980)
  3. Sheller et. al, 2005 European Journal of Biochemistry (2005)
  4. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  5. Celis JE, Cell biology: a laboratory handbook v2, Elseveir Academic Press, 2006 pp.161
  6. Perutz, Science is not a quiet life: Unravelling the atomic mechanism of haemoglobin, World Scientific Publishing, 1997
  7. Kebede N, Francis PS, Barbante GJ, Hogan CF, Electrogenerated chemiluminescence of tris (2,2’ biopyridine) ruthenium (II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte, Royal Society of Chemistry 2015(140) pp7142-7145