Biological Buffers


Synonym: 3-(Cyclohexylamino)-1-propanesulfonic acid

CAPS buffer is used extensively in Western and immunoblotting experiments as well as protein sequencing and identification. CAPS is used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. CAPS should be used to transfer proteins with a pl > 8.5 because of its high pH1.
Useful pH Range 9.7 - 11.1
Working concentration range 10mM3


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
CAPS 1135-40-6 C9H19NO3S 221.32 C2632 RDD015 239782 C6070 29337    


Interaction Comments

No reported interaction found


Literature References

  1. K J Ellis, J F Morrison. Buffers of constant ionic strength for studying pH-dependent processes. Methods Enzymol. 1982;87:405-26
  2. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  3. Gonzalez-Alvarez R, ADP-Ribosylation Reactions: From bacterial pathogenesis to cancer, Springer-Science+ business media LLC, 1999