Biological Buffers


Synonym: 2-(Cyclohexylamino)ethanesulfonic acid

CHES is a zwitterionic N-substituted aminosulfonic acid. CHES is used for studying pH-dependent processes in enzymology1.
Useful pH Range 8.6 - 10.0
Working concentration range 20mM-60mM5,6


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
CHES 103-47-9 C8H17NO3S 207.29 C2885 3100-OP C8210 29311 RES1299C-B7  


Interaction Comments

CHES interferes with the Lowry assay.
CHES has a high affinity for the iodoacetate binding site of liver alcohol dehydrogenase2.
Strong interaction with Mg, Ca, Mn, Co and Pb3


Literature References

  1. Ellis, K.J., et al. 1982. Meth. Enzymol. 87: 405-426. PMID: 7176924
  2. Syvertsen, C., et al. 1981. Eur. J. Biochem. 117: 165-170.
  3. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  4. Vallejo LF, Rinas U. 2012 FEBS journal 83-92
  5. Kaale E, Long Y, Fonge HA, Govaerts C, Desmet K, Schepdael AV, Hoogmartens J, Gentamicin Assay in human serum by solid-phase extraction and capillary electrophoresis, Electrophoresis 2005(26) pp. 640-647
  6. Reedijk J, Bouwman E –BioInorganic Catalysis—Marcel Dekker Inc 1999 pp. 51