Biological Buffers


Synonym: 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid)

HEPPS (EPPS) has many properties similar to HEPES. Because of its high buffer range, it is suited for studies on phosphorylation reactions when metal binding is a concern1. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase3. Its chemical structure contains a piperazine ring.
Useful pH Range 7.3 - 8.7
Working concentration range 10mM-1M5,6


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
HEPPS (EPPS) 16052-06-5 C9H20N2O4S 252.33 E9502 E0276 E1894 54465    


Interaction Comments

HEPPS (EPPS) interferes with the Folin protein assay, but not with the Biuret assay2
Slight interaction with Ni and Pb4


Literature References

  1. Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
  2. Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.
  3. P.Gill, J.G. Sutton Electrophoresis 6, 23, (1985)
  4. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  5. Gupta B, Taha M, Lee M-J, Buffers more than buffering agent: introducing a new class of stabilizers for the protein BSA, Phys.Chem.Chem.Phys 2015(17) pp 1114-1133
  6. Kebede N, Francis PS, Barbante GJ, Hogan CF, Electrogenerated chemiluminescence of tris (2,2’ biopyridine) ruthenium (II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte, Royal Society of Chemistry 2015(140) pp7142-7145