Biological Buffers


Synonym: Piperazine-N,N′-bis(2-ethanesulfonic acid)

PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer. PIPES has a pKa near the physiological pH which makes it useful in cell culture work1. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues2,3.
Useful pH Range 6.1 - 7.5
Working concentration range 20mM6 


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
PIPES 5625-37-6 C8H18N2O6S2 302.37 528131 P6757  RDD004 P8203 80635
PIPES sodium salt 10010-67-0 C8H17N2O6S2Na 324.35 P2949       
PIPES disodium salt 76836-02-7 C8H16N2Na2O6S2 346.33 P3768   RES0704P-A7  
PIPES dipotassium salt 108321-27-3 C8H16K2N2O6S2 378.55 P7643      
PIPES sesquisodium salt 100037-69-2 C8H16.5N2Na1.5O6S2 335.34 P8655 6910-OP      


Interaction Comments

PIPES has a slight capacity to bind divalent ions4.
Slight interaction with Co and Ni5


Literature References

  1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
  2. Salema, R. and Brandao, I., J. Submicr. Cytol., 9, 79 (1973)
  3. Schiff, R.I., et al. 1979. Scan Electron Microsc. 449-458. PMID: 118516
  4. Blanchard, J.S. 1984. Meth. Enzymol. 104: 404-414. PMID: 6717292
  5. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  6. Downs SM, Mastropolo AM, Culture Conditions Affect Meiotic Regulation in Cumulus Cell-Enclosed Mouse Oocytes, Molecular Reproduction and Development 1997(46) 551-566