Biological Buffers


Synonym: 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]-2-hydroxypropane-1-sulfonic acid or 3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid

TAPSO is a zwitterionic biological buffer and is one of the Good's buffers that is useful in cell culture media formulations1.
Useful pH Range 7.0 - 8.2
Working concentration range 20mM-50mM3,4


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
TAPSO 68399-81-5 C7H17NO7S 259.28 T9269      
TAPSO sodium salt 105140-25-8 C7H16NO7SNa 281.26 T5395       


Interaction Comments

Strong interaction with Mg, Ca, Fe, Cu and Pb. Slight interaction with Mn, Co, Ni, Zn and Cd2


Literature References

  1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
  2. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  3. Al-Ghosbashy MA, Electrophoretic behavior of charge regulated zwitter ionic buffers in covalently and dynamically coated fused silica capillaries, Bulletin of Faculty of Pharmacy. 2014(52) pp. 71-78
  4. Petriska I, Polasek M, Sladkowski R, Assay of terpene alcohols in pharmacopoeial essential oils by micellar electrokinetic capillary chromatography (MEKC), Pharmzie 2006(61) pp.989-993