Biological Buffers


Synonym: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid

Because the pKa is 7.4 (physiological pH), TES has potential for a variety of biological applications. In many culture media that require metal cations, many buffers cannot be used due to chelation or precipitation (citrate or phosphate, for example). TES was found to be beneficial in a study of succinate oxidation1. TES is a suitable buffer for epidermal cell growth at pH 7.4 - 7.9 where additional buffering capacity is required2.
Useful pH Range 6.8 - 8.2
Working concentration range 50mM-560mM6


        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grade Life Science Grades PharmaGrade Solution
TES 7365-44-8 C6H15NO6S 229.25 T1375  5.05147 T5691 T6541 93359    
TES sodium salt 70331-82-7 C6H14NNaO6S 251.23 T0772       
TES hemisodium salt   C6H14.5NO6S • 0.5Na 240.24 T1030      


Interaction Comments

TES can serve as a stabilizer for the globular protein BSA, in aqueous solutions3
Strong interaction with Cr and Fe; weak interaction with Co, Ni, Cu and Zn



  1. Good, N.E., et al., Biochemistry, v. 5, 467 (1966).
  2. Hammar, H. and Halprin, K., Epidermis Dis., Proc. Eur. Soc. Dermatol. Res. Symp., 1979, 243 (1981).
  3. Gupta, Bhupender S.; Taha, Mohamed; Lee, Ming-Jer. 2013. Process Biochemistry. 48.11: 1686-1696.
  4. Product Information Sheet
  5. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  6. Bartlett JMS, Stirling D, Methods in molecular biology 2nd edition Humana Press, 2003