Biological Buffers

Trizma® (TRIS base)


  • 2-Amino-2-(hydroxymethyl)-1,3-propanediol,
  • THAM,
  • Tris base,
  • Tris(hydroxymethyl)aminomethane,
  • Trometamol
TRIS is an established basimetric standard and buffer used in biochemistry and molecular biology1. It is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. TRIS buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
Useful pH Range 7 - 9
Working concentration range 10-62.5mM9,10


        Comparison of products by grade or quality level  
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
TRIZMA® base 77-86-1 C4H11NO3 121.14 T4661 RDD008 93352 T1503 T6066 93362 T6791   T1669
Tromethamine 77-86-1 C4H11NO3 121.14     RES3137T-A7 RES3193T-A7  
Sigma 7-9® 77-86-1 C4H11NO3 121.14 T1378      
Trizma® hydrochloride / TRIS hydrochloride 1185-53-1 C4H12ClNO3 157.60 RDD009 T3253 9310-OP 648313 93363 T5941 T6666 648317 RES3098T-B7 PHG0002  


Interaction Comments

TRIS may form Schiff bases with aldehydes and ketones.5
TRIS has been reported to interfere with the activity of some enzymes; it should, therefore, be used with caution when studying proteins2,3.
TRIS exhibits a substantial shift in dissociation with a change in temperature.6
TRIS interferes with the Bradford dye-binding protein assay4
TRIS has a strong interaction with Cr, Fe, Co, Ni and Cu; and, a weak interaction with Mg, Ca, Zn, Cd and Pb8


Literature References

  1. Gomori, G., Preparation of Buffers for Use in Enzyme Studies. Methods Enzymol., 1, 138-146 (1955).
  2. Desmarais, W.T., et al. 2002. Structure. 10: 1063-1072. PMID: 12176384
  3. Ghalanbor, Z., et al. 2008. Protein Pept. Lett. 15: 212-214. PMID: 18289113
  4. Stoll, V.S. and Blanchard, J.S. (1990) Buffers: Principles and practice. Meth. Enzmol. 182, 24–38.
  5. Hermanson, G.T. (1996). Bioconjugate Techniques, Academic Press.
  6. El-Harakany, A.A.; Abdel Halima, F.M. and Barakat, A.O. (1984). "Dissociation constants and related thermodynamic quantities of the protonated acid form of tris-(hydroxymethyl)-aminomethane in mixtures of 2-methoxyethanol and water at different temperatures". J. Electroanal. Chem. 162 (1–2): 285–305.
  7. Mohamed Taha, Ming-Jer Lee. Biochemical Engineering Journal. Volume 46, Issue 3, 1 November 2009, Pages 334–344.
  8. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  9. Western blot protocol- Duke University
  10. Shepherd LD, McLay TGB, Two micro-scale protocols for the isolation of DNA from polysaccharide-rich plant tissue, J Plant Res 2011 Mar;124(2):311-4.