Custom DNA and RNA Oligos & qPCR Probes Learning Center


Beginning May 8, 2022, BHQ® (Black Hole Quencher®) 1 and 2 will no longer be available as a modification to Custom DNA Oligos and Custom qPCR Probes. The replacements will be BXQ™ (Block All Quencher™) 1 and 2. Information about the BXQ dark quenchers will be added from time-to-time to key oligo landing pages up until the date of change. As these key landing pages are updated, this message will be updated to provide links to the new information. If you have any questions, please contact your local company representative. Alternatively, to learn more about BXQ, please visit the manufacturer’s website:  BXQ | AAT Bioquest.


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OligoEvaluator™ Calculator

The OligoEvaluator™ oligonucleotide sequence calculator provides oligo analysis values.

DNA Oligonucleotide Synthesis

Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing.

Protocol for Annealing Oligonucleotides

The annealing process is divided into two main steps: 1) dissolution, and 2) annealing, which are performed by either heat block or thermocycler. Although each oligonucleotide is provided in a specific amount, for best results, measure the OD before use.

 Custom DNA and RNA Oligos & qPCR Probes portfolio: Discover More