Functional Genomics & RNA

Trophoblast Protocol

Trophoblast Protocol Product lists for commonly used protocols from Cold Spring Harbor Protocols


Culturing Trophoblast Stem (TS) Cell Lines


Janet Rossant
This protocol was adapted from "Isolation and Culture of Blastocyst-Derived Stem Cell Lines," Chapter 8 (Protocol 8, provided by the laboratory of Janet Rossant), in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

Recently, culture conditions have been established for a second blastocyst-derived cell line, trophoblast stem (TS) cells, in addition to embryonic stem (ES) cells. This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.
Manipulating the Mouse Embryo - Third Edition


Products Available for this Protocol
Protocol Material Description Product # Product Name Add to Cart
Dimethylsulfoxide D2650 Dimethyl sulfoxide, liquid (temperature dependent), ≥99.7%, Hybri-Max™, sterile-filtered, hybridoma tested
Heparin H4784 Heparin sodium salt, ~170 USP units/mg
Gelatin (Swine Skin) G2500 Gelatin from porcine skin, Type A
Trypsin-EDTA T3924 Trypsin-EDTA solution, 1 ×, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks' Balanced Salt Solution with phenol red., sterile-filtered, cell culture tested
BSA fraction V A4503 Albumin from bovine serum, Cohn V fraction, ≥96% (agarose gel electrophoresis), lyophilized powder
PBS P5368 Phosphate buffered saline, pH 7.4, Biotechnology Performance Certified
Mitomycin C stock M4287 Mitomycin C from Streptomyces caespitosus, powder, cell culture tested
Dulbecco’s modified Eagle’s medium (DMEM) D6046 Dulbecco’s Modified Eagle’s Medium - low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, cell culture tested
Fetal bovine serum (FBS) F2442 Fetal Bovine Serum, USA origin, sterile-filtered, cell culture tested, hybridoma tested
Sodium pyruvate P5280 Sodium pyruvate, cell culture tested, insect cell culture tested, ≥99%, powder
β-mercaptoethanol M3148 2-Mercaptoethanol, for molecular biology, ≥98% (GC/titration)
L-glutamine G6392 L-Glutamine, γ-irradiated, cell culture tested
Penicillin and Streptomycin P4333 Penicillin-Streptomycin, 100 ×, liquid, stabilized, sterile-filtered, cell culture tested
RPMI 1640 R8758 RPMI-1640 Medium, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, cell culture tested

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Product Association Disclaimer: The products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. Our products/reagents have been qualified for use, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.