Functional Genomics & RNAi

Predesigned miRNA Mimics

All ordering options are in the miRNA Mimic Product Options & Specifications table.

MISSION® predesigned miRNA (microRNA) Mimics mimic endogenous, mature miRNA when transfected into cells. Most miRNA are known to knockdown gene expression in a variety of manners, including by translational repression, mRNA degradation, and endonucleolytic cleavage.


 Product Benefits

  • Functionally tested for knockdown efficiency against natural targets
  • Unique design significantly reduces possible sense strand off-target effects
  • Distinguish sequence-specific silencing from non–specific effects with 2 negative control miRNA

 Product Features

  • Purification: Desalt
  • Sequence Form: 21mer duplexes with 1 mismatch and overhangs
  • Modifications: 5' amine on sense strand
  • Quality Control: 100% mass spectrometry

For general inquiries regarding this product, please send a request to

Browse the complete list of predesigned miRNA Mimics.

miRNA Mimic Product Options & Specifications
Individual miRNA Mimic • Definition: Individual, predesigned
  miRNA for various targets
• Species: Human
• Quantities: 1 (5 nmol) OD
• Format: Dry in tubes
hsa-000-000 to hsa-mir-219

hsa-mir-220 to hsa-miR-545

hsa-miR-546 to hsa-miR-1200

hsa-miR-1201 to hsa-miR-3200

hsa-miR-3201 to hsa-miR-4520

hsa-miR-4521 to hsa-miR-4800

hsa-miR-4801 to hsa-miR-6800

hsa-miR-6801 to hsa-miR-8089

miRNA Mimic Library


• Definition: Predesigned miRNA for all
  targets in current version of miRBase
• Species: Human
• Quantities: 0.25 nmol
• Format: Dry in 96-well plates
  (inquire for 384-well)


 Control miRNA

Two negative controls are available to distinguish sequence-specific silencing from non-specific effects:

 Validation Data

Our predesigned miRNA Mimics significantly reduce known gene target expression (Figure 1).

Figure 1. HeLa cells were co-transfected with predesigned miRNA Mimics and psi-CHECK2 Dual Luciferase Reporter Construct (Promega), containing corresponding miRNA target sequences. Constructs contained full-length 3'UTR sequence-specific miRNA sequences (100% complementary to the mature miRNA).

In addition, possible sense strand off-target effects are minimal (Figure 2).

Figure 2. Passenger strands of miRNA mimic for miR-206 was either designed by traditional siRNA design (siDesign) with (+) or without (-) chemical modification, or by our miRNA Mimic Design software with (+) or without (-) chemical modification. Reporter constructs contained targets that were ~95% complementary to the passenger strand sequence. Our proprietary design and chemical modification effectively reduces off-target effects.


 Select Citations

  • Humphreys DT, Hynes CJ, Patel HR, Wei GH, Cannon L, Fatkin D, Suter CM, Clancy JL & Preiss T (2012). Complexity of murine cardiomyocyte miRNA biogenesis, sequence variant expression and function. PLoS One, 7(2), e30933.
  • Sun L, Zhang D, Liu F, Xiang X, Ling G, Xiao L, Liu Y, Zhu X, Zhan M, Yang Y, Kondeti VK, & Kanwar YS (2011). Low-dose paclitaxel ameliorates fibrosis in the remnant kidney model by down-regulating miR-192. J Pathol, 225, 364-377.
  • Schultz N, Marenstein DR, De Angelis DA, Wang WQ, Nelander S, Jacobsen A, Marks DS, Massagué J & Sander C (2011). Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2. Silence, 2, 3.
  • Collino F, Deregibus MC, Bruno S, Sterpone L, Aghemo G, Viltono L, Tetta C & Camussi G (2010). Microvesicles Derived from Adult Human Bone Marrow and Tissue Specific Mesenchymal Stem Cells Shuttle Selected Pattern of miRNAs. PLoS One, 5(7), e11803.

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MISSION is a trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. Label License.