Functional Genomics & RNAi

Predesigned miRNA Mimics

All ordering options are in the miRNA Mimic Product Options & Specifications table.

MISSION® predesigned miRNA (microRNA) Mimics mimic endogenous, mature miRNA when transfected into cells. Most miRNA are known to knockdown gene expression in a variety of manners, including by translational repression, mRNA degradation, and endonucleolytic cleavage.

 

 Product Benefits

  • Functionally tested for knockdown efficiency against natural targets
  • Unique design significantly reduces possible sense strand off-target effects
  • Distinguish sequence-specific silencing from non–specific effects with 2 negative control miRNA

 Product Features

  • Purification: Desalt
  • Sequence Form: 21mer duplexes with 1 mismatch and overhangs
  • Modifications: 5' amine on sense strand
  • Quality Control: 100% mass spectrometry

For general inquiries regarding this product, please send a request to sirnarequest@sial.com

Browse the complete list of predesigned miRNA Mimics.
 

miRNA Mimic Product Options & Specifications
Individual miRNA Mimic • Definition: Individual, predesigned
  miRNA for various targets
• Species: Human
• Quantities: 1 (5 nmol) OD
• Format: Dry in tubes
 
hsa-000-000 to hsa-mir-219


hsa-mir-220 to hsa-miR-545


hsa-miR-546 to hsa-miR-1200


hsa-miR-1201 to hsa-miR-3200


hsa-miR-3201 to hsa-miR-4520


hsa-miR-4521 to hsa-miR-4800


hsa-miR-4801 to hsa-miR-6800


hsa-miR-6801 to hsa-miR-8089

miRNA Mimic Library

 

• Definition: Predesigned miRNA for all
  targets in current version of miRBase
• Species: Human
• Quantities: 0.25 nmol
• Format: Dry in 96-well plates
  (inquire for 384-well)
     

 

 Control miRNA

Two negative controls are available to distinguish sequence-specific silencing from non-specific effects:

 Validation Data

Our predesigned miRNA Mimics significantly reduce known gene target expression (Figure 1).

Figure 1. HeLa cells were co-transfected with predesigned miRNA Mimics and psi-CHECK2 Dual Luciferase Reporter Construct (Promega), containing corresponding miRNA target sequences. Constructs contained full-length 3'UTR sequence-specific miRNA sequences (100% complementary to the mature miRNA).

In addition, possible sense strand off-target effects are minimal (Figure 2).

Figure 2. Passenger strands of miRNA mimic for miR-206 was either designed by traditional siRNA design (siDesign) with (+) or without (-) chemical modification, or by our miRNA Mimic Design software with (+) or without (-) chemical modification. Reporter constructs contained targets that were ~95% complementary to the passenger strand sequence. Our proprietary design and chemical modification effectively reduces off-target effects.

 

 Select Citations

  • Humphreys DT, Hynes CJ, Patel HR, Wei GH, Cannon L, Fatkin D, Suter CM, Clancy JL & Preiss T (2012). Complexity of murine cardiomyocyte miRNA biogenesis, sequence variant expression and function. PLoS One, 7(2), e30933.
  • Sun L, Zhang D, Liu F, Xiang X, Ling G, Xiao L, Liu Y, Zhu X, Zhan M, Yang Y, Kondeti VK, & Kanwar YS (2011). Low-dose paclitaxel ameliorates fibrosis in the remnant kidney model by down-regulating miR-192. J Pathol, 225, 364-377.
  • Schultz N, Marenstein DR, De Angelis DA, Wang WQ, Nelander S, Jacobsen A, Marks DS, Massagué J & Sander C (2011). Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2. Silence, 2, 3.
  • Collino F, Deregibus MC, Bruno S, Sterpone L, Aghemo G, Viltono L, Tetta C & Camussi G (2010). Microvesicles Derived from Adult Human Bone Marrow and Tissue Specific Mesenchymal Stem Cells Shuttle Selected Pattern of miRNAs. PLoS One, 5(7), e11803.

If additional help is needed, please consult our technical services group at oligotechserv@sial.com.

MISSION is a trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. Label License.