Functional Genomics & RNAi

Predesigned miRNA Inhibitors

All ordering options are in the miRNA Inhibitor SKUs table.

MISSION® Predesigned miRNA inhibitors regulate gene expression by binding to and inhibiting specific mature miRNA. The miRNA inhibitors were designed using a proprietary algorithm and the mature miRNA sequence information from miRBase. The algorithm computes all possible sequence parameters, selects the one predicted to best maintain the sTuD (Synthetic Tough Decoy) structure, providing maximum miRNA recognition and binding. Each sTuD—stabilized by 2’-O-methylated nucleotides—provides two miRNA binding sites.

 

 Product Benefits

  • Excellent resistance to nucleases
  • Potent inhibition of target miRNA at low concentrations
  • Distinguish sequence-specific silencing from non–specific effects with 2 negative control miRNA

 

 Product Features

  • Species: Human, Mouse & custom (inquire)
  • Quantities: 1 (5 nmol) OD
        • 20 nmol for custom
  • Purification: Desalt
  • Sequence Form: 21mer duplexes
  • Modifications: 2’-OMe-RNA
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in tubes

For general inquiries regarding this product, please send a request to sirnarequest@sial.com.

Browse the complete list of predesigned miRNA Inhibitors.

 

miRNA Inhibitor SKUs
Individual human miRNA Inhibitors
Individual mouse miRNA Inhibitors
Custom miRNA Inhibitors  

 

 Control miRNA Inhibitors

Two negative controls are available to distinguish sequence-specific silencing from non-specific effects:

 

 Validation Data

Our predesigned miRNA Inhibitors significantly reduce miRNA-mediated gene knockdown (Figure 1).

 

HeLa cells were co-transfected with predesigned miRNA Inhibitors

Figure 1. HeLa cells were co-transfected with predesigned miRNA Inhibitors and a psi-CHECK2 Dual Luciferase Reporter Construct (Promega), containing corresponding miRNA target sequences, 100% complementary to the mature miRNA, downstream from Renilla. Increased Renilla expression is observed when endogenous miRNA activity is inhibited. The miRNA inhibitor negative control targets Arabidopsis miRNA 416, which is not expressed in human cells.

Predesigned miRNA Inhibitors effectively target miRNA activity across a wide concentration range (Figure 2).

HeLa cells were co-transfected with predesigned miRNA Inhibitors

Figure 2. HeLa cells were co-transfected with predesigned miRNA Inhibitors and a psi-CHECK2 Dual Luciferase Reporter containing 2 copies of the sequence, 100% complementary to the corresponding mature miRNA, downstream from Renilla. Increased Renilla expression is observed when endogenous miRNA activity is inhibited. The miRNA inhibitor negative control targets Arabidopsis miRNA 416, which is not expressed in human cells. Optimum inhibitor concentration should be determined empirically for each specific experimental situation.

Predesigned miRNA Inhibitors exhibit activity equivalent or superior to competing technologies (Figure 3).

HeLa cells were co-transfected with predesigned miRNA Inhibitors

Figure 3. HeLa cells were co-transfected with an inhibitor from each company and a psi-CHECK2 Dual Luciferase Reporter with containing 2 copies of the sequence, 100% complementary to the corresponding mature miRNA, downstream from Renilla. Increased Renilla expression is observed when endogenous miRNA activity is inhibited. Our product equals are exceeds those from the competition.

 

 Select Citations

  • Haraguchi T, Nakano H, Tagawa T, Ohki T, Ueno Y, Yoshida T & Iba H (2012). A potent 2'-O-methylated RNA-based microRNA inhibitor with unique secondary structures. Nucleic Acid Res, 40, e58.
  • Haraguchi T, Ozaki Y & Iba H (2009). Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acid Res, 37, e43.

If additional help is needed, please consult our technical services group at oligotechserv@sial.com.

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