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New Applications Data, Protocols and Publications


Bioactive Small Molecules Literature References

Video Protocol: "Mission esiRNA for RNAi Screening in Mammalian Cells"

Mashimo, T. et al. Generation of Knockout Rats with X-Linked Severe Combined Immunodeficiency (X-SCID) using Zinc-Finger Nucleases. PLos One 2010 Jan 25;5(1):433e8870 (2.2 Mb PDF)

ZFN applications on stem cells research and the creation of the first knock-out rat.
Jaenish, R. et al. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat. Biotechnol. 2009 Sep;27(9):851-7. Epub 2009 Aug 13

Buelow, R. et al. Knockout rats via embryo microinjection of zinc-finger nucleases. Science 2009 Jul 24;325(5939):433 (Abstract)

Carlson, M.E. et al. Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells. Nature 2008 July 24, 454(7203), 528-32. (Abstract)

Doyon, Y. et al. Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat. Biotechnol. 26(6), 702–708 (2008). (340 Kb PDF)

Meng, W. et al. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell 2008 Nov 28, 135(5), 948-59. (Abstract)

Duan, Z. et al. Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresitance in human ovarian cancer. Mol. Cancer. Ther. 7, e-publication, 2008. (Abstract)

Brahmamdam, P., et al. Targeted delivery of siRNA to cell death proteins in sepsis. Shock Nov 11 2008 (Epub ahead of print)

For our complete list of RNAi references, visit our RNAi Bibliographies page


Spinoculation, a method of enhancing lentiviral transduction efficiency
Reverse Transfection Using N-TER/siRNA Nanoparticles (508 Kb PDF)
N-TER/siRNA nanoparticle mediated knockdown of gene expression—HUVEC Cells (476 Kb PDF) 

For additional protocols visit the protocols page


MISSION TRC shRNA library Validation (292 Kb PDF)
Precision Genome Editing in Mammalian Cells Using Engineered Zinc Finger Proteins (839 Kb PDF)


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