MISSION® siRNA

in vivo FAQs

Do you have any data for in vivo quality siRNA?
Yes, we do. Sigma-Aldrich teamed up with Bioo Scientific™ to validate our MISSION In Vivo Quality siRNA with Bioo Scientific's MaxSuppressor™ In Vivo RNA-LANCEr II in vivo delivery agent. Please visit our website, sigma.com/invivosirna for details of this collaboration. In addition, please consult PubMed to identify similar studies to determine the specific delivery needs.
 
Do you have siRNA that is specifically for in vivo experiments?
Yes, Sigma-Aldrich does offer MISSION In Vivo Quality and iScale Oligos siRNA. For the In Vivo Quality siRNA specifications, please see /life-science/functional-genomics-and-rnai/sirna/in-vivo-quality-specs.html. Please note that specifications on large scale siRNA may vary. iScale Oligos siRNA is "intermediate scale" siRNA, and it is available in standard desalt or HPLC purification so that researchers may order larger quantities of siRNA for other areas of research besides in vivo work.
 
How long does siRNA-mediated knockdown last in vivo?
Please see the literature for this information. We have several In Vivo references, with links to the abstracts, on our website at /life-science/functional-genomics-and-rnai/learning-center/rnai-bibliographies.html#in%20vivo%20applications. In addition, please consult PubMed to identify similar studies to determine the specific delivery needs.
 
How much siRNA do I need for in vivo experiments in mice?
This can depend on the delivery reagent you choose. Please refer to the supplier’s technical bulletin for the protocol.
 
Is siRNA toxic in vivo?
Please see the literature for this information. We have several In Vivo references, with links to the abstracts, on our website at /life-science/functional-genomics-and-rnai/learning-center/rnai-bibliographies.html#in%20vivo%20applications. In addition, please consult PubMed to identify similar studies to determine the specific delivery needs.
 
What factors should I address before initiating an in vivo study?
You need to outline your experimental design for in vivo studies. This includes the choice of model organism, route of delivery of siRNA, whether or not to use a delivery reagent, dosage, and frequency of dosing. The concentration delivered will depend largely on the nature of the target, the expression pattern, and the size of the study.
 
What tissues have siRNAs been delivered into?
Please see the literature for this information. We have several In Vivo references, with links to the abstracts, on our website at /life-science/functional-genomics-and-rnai/learning-center/rnai-bibliographies.html#in%20vivo%20applications. In addition, please consult PubMed to identify similar studies to determine the specific delivery needs.
 
What controls should I use with an in vivo siRNA experiment?
Please see the literature for this information. We have several In Vivo references, with links to the abstracts, on our website at /life-science/functional-genomics-and-rnai/learning-center/rnai-bibliographies.html#in%20vivo%20applications. In addition, please consult PubMed to identify similar studies to determine the specific delivery needs.
 
What delivery reagent should I use for siRNA in vivo?
There are several delivery reagents on the market. The delivery reagent you choose depends on the target organ(s). Please refer to the supplier's technical bulletin for target organ information. Please see the literature for this information. We have several In Vivo references, with links to the abstracts, on our website at /life-science/functional-genomics-and-rnai/learning-center/rnai-bibliographies.html#in%20vivo%20applications. In addition, please consult PubMed to identify similar studies to determine the specific delivery needs.