siRNA

Predesigned Guarantee

 

At least two of three purchased MISSION® Predesigned siRNA targeting the same gene will reduce target mRNA levels by 75%, in cultured cells, when transfected at a concentration of ≥30 nM. If two of the siRNA do not knock down the target gene by 75%, we will provide three additional siRNA for that gene, free of charge. If there are no more siRNA for that gene or all siRNA fail to knock down the target gene by 75%, we will refund the purchase price.

Receipt of appropriate supporting data for transfection efficiency is required for the guarantee to apply. Appropriate supporting data for transfection efficiency would include qPCR data comparing target mRNA levels of a MISSION Positive Control siRNA (GAPDH, MAPK1, TP53, etc.), transfected at ≥30 nM, to an appropriate negative control (such as mock transfection, a scrambled siRNA sequence, or MISSION Universal Negative Control siRNA), demonstrating knockdown of the target mRNA of 75%.

Due to the variability of antibodies and protein half-lives, we are unable to accept data from protein-based detection methods.