Attention:

Certain features of sigmaaldrich.com will be down for maintenance Saturday afternoon/evening, February 24th starting at 3:00 pm CT until 9:00 pm CT.

Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Functional Genomics & RNAi

TargeTron® Gene Knockout System

Genetic Engineering that is Right on Target!

Introduction to TargeTron

The TargeTron Gene Knockout System (TA0100) is a revolutionary method for rapid and specific disruption of genes in prokaryotic organisms. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches.


Features

  • Targeted and permanent gene disruption
  • Simple, streamlined protocol; Knockouts in 3 days or less
  • Minimal screening to isolate mutants
  • >90% successful targeted insertion
  • No specific host factor requirements
  • To pre-screen your target sequence, please contact Technical Service.

Resources
TargeTron Vectors
Detailed Description and Algorithm
User Guide (PDF)
pACD4K-C Vector Sequence (TXT)
Updated License Agreement (PDF)
Frequently Asked Questions
References
Ordering Information

Host Range
TargeTron has been validated in multiple organisms; however, work to adapt the system to other organisms is ongoing and data will be provided as it becomes available.

Related Products
Below is an easy guide to materials needed for or that complement the TargeTron Gene Knockout System.

Related Technologies
Functional Genomics

This product and its use are the subject of one or more of U.S. Patent Nos. 5,698,421, 5,804,418, 6,027,895 and 6,001,608.
 

   TargeTron Design Request Form

TargeTron is currently on production hold while we re-evaluate how to best serve our bacterial genetic engineering customers. For customers who have previously-purchased TargeTron products, please click here for intron-retargeting primer design services.
 

Organism Reference
Agrobacterium tumefaciens Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector
Bacillus subtilis (TargeTron Vector pNL9164, Product No. T6701)
Clostridium acetobutylicum The ClosTron: A universal gene knock-out system for the genus Clostridium
Clostridium botulinum The ClosTron: A universal gene knock-out system for the genus Clostridium
Clostridium difficile The ClosTron: A universal gene knock-out system for the genus Clostridium
Clostridium perfringens (TargeTron Vector pJIR750ai, Product No. T7701) Disruption of a toxin gene by introduction of a foreign gene into the chromosome of Clostridium perfringens using targetron-induced mutagenesis
Clostridium sporogenes The ClosTron: A universal gene knock-out system for the genus Clostridium
Escherichia coli (TargeTron Gene Knockout System, Product No. TA0100)
Lactococcus lactis (TargeTron Gene Knockout System, Product No. TA0100)
Proteus mirabilis Reference in press
Pseudomonas aeruginosa Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector
Salmonella typhimurium (TargeTron Gene Knockout System, Product No. TA0100)
Serratia marcescens Unpublished to date
Shigella flexneri (TargeTron Gene Knockout System, Product No. TA0100)
Staphylococcus aureus (TargeTron Vector pNL9164, Product No. T6701)
Xylella fastidiosa Unpublished to date