Functional Genomics & RNAi


The TargeTron Vector pNL9164 (T6701) is a circularized, expression vector derived from pNL91621. The vector can be used for targeted gene knockouts in gram-positive organisms such as Staphylococcus aureus1. As supplied, the pNL9164 vector is retargeted to the hsa gene in S. aureus. For validation in S. aureus, this vector can be used directly to knockout hsa without any further modifications (protocol included). In order to retarget this vector to other genes, please refer to pages 6-9 in the TA0100 User Guide (1.1 MB PDF).

Technical Bulletin

Vector Map

Ordering Information


  1. Yao, J. et. al, RNA. (2006) (in press).


Related Products
TargeTron Gene Knockout System

TargeTron Vector

pNL9164 Vector Map

TargeTron is a registered trademark of InGex, LLC.

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TargeTron® Gene Knockout System