MILLIPLEX® Multiplex Assays for Luminex® FAQs

 

MILLIPLEX® Multiplex Product Catalog and Information

For the most current and comprehensive catalog of MILLIPLEX® products, download a copy of our Analyte Quarterly to see our entire portfolio of MILLIPLEX® Assays, Luminex® Instruments, and all of our immunoassay solutions and offerings, from Single Molecule Counting (SMC™) technology to ELISA kits.

Additionally, our Tips & Tricks brochure provides technical information on MILLIPLEX® assays and Luminex® instruments, including information on the technology and how it works, protocols, data analysis and species cross-reactivity data for our most popular analytes.

  1. Who do I contact if I need additional assistance about an order, product price and availability, or login assistance?
    Please contact our Customer Service by phone.

  2. Who do I contact if I have technical or scientific questions or concerns about a MILLIPLEX® Assay?
    Please follow the link to contact Technical Support by phone or send a message to Technical Support.

  3. Who do I contact if I need to request a call or visit from my Biomarker Sales Specialist?
    To request a call or visit from your Biomarker Sales Specialist please send a message to the Sales Team.

  4. Who can I contact if I need to replace a reagent in a MILLIPLEX® Kit?
    Please contact Technical Support, Customer Service, or your Sales Representative.

  5. Where can I find more information if I am planning a large, long-term study or writing a grant proposal for funding?
    Request a Sales Specialist to contact you to assist with your immunoassay needs, including assay validation requirements, custom assays, supply chain planning, quality agreements, packaging and shipping, and reagent change notifications.

  6. Where can I find more information about your Custom Assays for new analytes or to combine analytes for multiple MILLIPLEX® Kits?
    For a custom MILLIPLEX® assay or ELISA, please visit our Custom Assays page or request information through the online form. If you’re interested in SMC® assays, visit our SMC® Custom Assays and Testing Services page.

  7. What kind of quality and assay verification do you provide with your MILLIPLEX® kits?
    MILLIPLEX® Kits are manufactured in facilities which are ISO 9001-2015 compliant and are for research use only (RUO), and not for use in clinical or for medical diagnostic purposes. Discover how our quality differentiates our MILLIPLEX® Assays from development to manufacturing.

  8. Are your MILLIPLEX® kits and analyte multiplexing kits customizable?
    Most of our MILLIPLEX® kits are customizable, often referred to as “configurable,” which means you may choose one, or any number or all of the analytes listed for your research and you only pay for the analytes you select.

  9. Can I mix reagents from more than one of the same type of kit?
    Please only mix reagents if the kit reagents are all from the same lot (lot numbers are on each bottle label inside the kit) and the reagents have been stored under the recommended conditions.

  10. Can two or more MILLIPLEX® kits be combined?
    Each kit is developed and verified to optimally perform with the included reagents and according to protocol recommendations. Combining kits can risk antibody cross-reactivity, incompatible sample dilution factors, inappropriate serum matrix, buffers or incubation periods – any of which can skew your results. If you wish to combine analytes from different kits, check out our Custom Assays.  You can rely on our expertise to help you find the best options for your research needs.

  11. Can I use a kit meant for one species to detect samples from another species?
    Our Tips and Tricks brochure contains an appendix listing analyte species cross-reactivity data.  You may also contact our Technical Support team.

  12. Can a MILLIPLEX® kit be used to detect an analyte in saliva, urine or tears?
    Our kits have been tested in the sample types indicated in each kit protocol. Typically, our kits are tested for use with plasma or serum samples, cell culture samples or lysate samples. Some of our kits have been specifically tested for urine or CSF (cerebral spinal fluid). Before trying our kits with a new sample type, please contact Technical Support for recommendations or our Custom Assays team can run verification testing on your specific sample type.

  13. How does the price of multiplexing compare to running ELISAs?
    Each MILLIPLEX® kit has a base price for the kit with one analyte. Each additional analyte adds incrementally to the price. If you only wish to detect one or two analytes, two separate single analyte ELISA kits may suit your budget and research needs. However, when you interrogate multiple analytes, multiplexing becomes a real cost-saver.

    If your goal is to save precious sample volume, then multiplexing is key. Most of our MILLIPLEX® kits require only 25 µL of sample, sometimes “neat” and sometimes diluted, according to each protocol. You save time and sample by running just one easy assay to quantify all your selected analytes of interest. Our kits come with all the reagents you need to complete your assay, including the assay plate and sealers. Just add water, sample and follow the protocol.

  14. Can I use any plate reader for MILLIPLEX® assays?
    A Luminex® instrument is required to run Luminex®-based immunoassays, including MILLIPLEX® assays and kits. There are three instruments currently available for use with MILLIPLEX® kits (or other Luminex partner’s kits): FLEXMAP 3D®, Luminex® 200™ and MAGPIX®. For more information on the differences between these instruments, visit our Luminex® Multiplexing Instruments page. These instruments may be purchased from us or from other Luminex partners. The Bio-Plex® instrument may also be used to run any Luminex®-based immunoassay including MILLIPLEX® assays.