Figure 4.
Reporter gene analysis of DRB1 promoter VDRE.
Raji B cells were transiently transfected with pGL3 luciferase constructs as indicated together with pRL_TK to normalise luciferase activity. Open bars indicate resting cells, grey shaded bars results following stimulation of transfected cells with 1,25-dihydroxyvitamin D3. Mean+/-SD of three independent transient transfection experiments are shown, each performed in quadruplicate. doi:10.1371/journal.pgen.1000369.g004