Figure 3d
VDR modulates β-catenin induced skin tumours. (A, B) K14ΔNβ-cateninER (D4) transgenic mice were treated with vehicle, 4-hydroxy-Tamoxifen (4OHT), EB1089 or 4OHT+EB1089 for 21 days. (A) Macroscopic appearance of tails. (B) Hematoxylin and eosin (H&E) stained tail skin sections. Arrows indicate parakeratosis and increased cornified layers. (C) H&E stained tail skin sections of wild type and K14ΔNβ-cateninER (D4) transgenic mice that were heterozygous (+/–) or homozygous (–/–) null for VDR, following 4OHT treatment for 21 days. Arrows indicate ectopic hair follicle formation. eHF: ectopic hair follicles; DP: dermal papilla; eDP: ectopic dermal papilla. (D, E) Human trichofolliculoma (D) and infiltrative basal cell carcinoma (E). Serial sections were stained with H&E or immunolabelled for β-catenin (red) and VDR (green) with Hoescht counterstain (blue). Immunolabelling corresponds to boxed regions of H&E stained sections. Arrowheads show nuclear β-catenin and VDR. Scale bars: 100 μm (B–E), 50 μm (inserts in d, e), 10 μm (Hoescht staining inserts d,e).Copyright© Palmer, H.G. et al., PLoS ONE, 3(1),e1483. This image is from an open access article distribution under the terms of the Creative Commons Attribution License.