Customer Education

MilliporeSigma Presents a Seminar at Georgetown University

Recent Advances in CRISPR/Cas9 Genome Editing Technology: From eSpCas9 to Whole Genome Screening

Seminar Title: Recent Advances in CRISPR/Cas9 Genome Editing Technology: From eSpCas9 to Whole Genome Screening

Date: Thursday, February 8, 2018

Time: Lecture 10:00 AM – 11:00 AM
Q&A 11:00 AM – 11:30 AM
Registration and Check-in: 9:45 AM

Location: Georgetown University NRB Auditorium



 Abstract

Genome editing has enabled the investigation of gene function and target identification, and is helping researchers develop potential therapies against a wide range of diseases. MilliporeSigma has been an industry leader in genome editing technology since 2002 when it introduced the TargeTron system for gene editing in prokaryotes. Five years later, it became the first company to commercialize eukaryotic genome editing technology in the form of Zinc Finger Nucleases (ZFNs). The company was at the forefront of the CRISPR revolution in 2012, and continues to develop cutting-edge genome and epigenome editing tools with ever greater target specificity and robust cutting activity.

After a brief introduction to CRISPR/Cas9 technology, this presentation will highlight the latest developments in the field of genome editing. We will discuss strategies to enhance homology-directed repair (HDR) to facilitate gene insertion or swap, including Cas9-Geminin. We will describe enhanced-specificity Cas9 (eSpCas9), which is a rationally engineered version of wild-type SpCas9 with significantly fewer off-target effects. We also will illustrate the proxy CRISPR targeting strategy, which makes CRISPR gene editing more efficient, flexible, and specific. We will explain epigenetic applications of CRISPR technology, including dCas9-p300 and CRISPR-SAM activators. We also will highlight pooled and arrayed CRISPR libraries, such as the widely-acclaimed Sanger LentiCRISPR array, which allows whole-genome or gene family screening. The presentation will end with a brief overview of other emerging technologies available from MilliporeSigma.

Topics that will be discussed include:

  1. CRISPR Background & Mechanism
  2. Recent CRISPR Innovations
        a. HDR enhancement
        b. SygRNA & eSpCas9
        c. Proxy CRISPR
  3. Exciting CRISPR Applications
        a. Epigenetic activators
        b. Whole genome lentiviral arrays & pools
  4. Emerging Technologies from MilliporeSigma
Speaker Bio
M. Zulfiquer Hossain, PhD
Research Technology Specialist
MilliporeSigma
Dr. Hossain received his BS in Biology and Biochemistry from Brandeis University and his PhD in Molecular Medicine from the University of Maryland under the Graduate Partnerships Program (GPP) with the National Institutes of Health (NIH). He has over 10 years of biomedical research experience, both as a pre-doctoral fellow at the NIH, where he discovered novel roles for β-catenin and TCF in T cell development, function, and aging, and as a post-doctoral fellow at the Johns Hopkins University, where he identified novel DNA-damaging polyphenols  in foods and flavorings. He brings extensive knowledge of molecular, cancer and stem cell biology, epigenetics and immunology, as well as teaching experience as an Associate Professor at BRAC University. As a Research Technology Specialist with MilliporeSigma, Dr. Hossain supports research projects that use cutting-edge functional genomics technologies.



For more information, please contact technicalworkshops@sial.com or:

Jill Clouse
Senior Research Sales Specialist
MilliporeSigma
Phone: 410-200-5397
Email: jill.clouse@sial.com