Customer Education

Webinar: Duolink® PLA Technology: How to detect and quantify protein interactions


 What Does it Cover?

Duolink® proximity ligation assay (PLA) technology allows you to visualize protein interactions with cellular localization and quantities by amplifying signals corresponding to single and post-translational protein events. With 1000x sensitivity and high specificity, this protein detection technology allows to visualize protein functions, all within a native cell. The PLA method provides:

  • Visual protein interactions
        − Both stable and transient
  • Endogenous protein detection
        − No overexpression or genetic manipulation
  • High specificity
        − Use of two antibodies/probes eliminates false positives
  • Single molecule sensitivity
        − Rolling circle amplification makes proteins visible
  • No special equipment needed
        − Standard immunofluorescence methods

This webinar will review how to work with PLA technology and provide an overview of their potential along with example applications.
 

 What Will You Learn?

  • How to work with Duolink® PLA technology
  • Overview of most relevant applications using Duolink®
  • Product offering to run an assay with PLA using Immunofluorescence or Flow Cytometry
  • Benefits of working with highly specific and powerful technology to study protein biology

 Who Should Attend?

Researchers in the T-Cell, adaptive immunotherapy, and immuno-oncology fields