Customer Education

Webinar: In Vitro Transporter Models for ADME-Tox Research

 

 What Does it Cover?

Drug transporters play a pivotal role in mediating the disposition of many drugs. As a result, researchers in the fields of drug discovery and development have shown a steadily rising level of interest in transporter-drug interactions, transporter-mediated drug-drug interactions, and the role of transporters in determining drug toxicity. For the past 18 years, SOLVO Biotechnology has pioneered the development and commercialization of in vitro assay systems to enable the study of drug transporters. One such system commonly employed for this purpose are inside-out membrane vesicles. Generated from cells over-expressing a transporter of interest, these membranes can be used to examine members of the ATP-Binding Cassette (ABC) transporter family, which includes P-glycoprotein (P-gp; MDR1), bile salt export pump (BSEP), breast cancer resistance protein (BCRP), and the multidrug resistance-associated proteins (MRPs). A versatile assay system, transporter-drug interactions can be monitored indirectly using ATPase measurements, or by directly measuring substrate transport using fluorescent or radiolabeled compounds, or by LC-MS detection. The advantages and limitations of this assay system will be discussed, and we will detail how inhibition of transporters involved in bile acid homeostasis is being used for predictive drug-induced liver injury (DILI) studies. In addition, we will examine the role that the lipid composition within the membrane plays on transporter activity within the context of the latest range of SOLVO mammalian membrane vesicles products for transporter research.
 

 What Will You Learn?

  • Role of transporters in ADME-Tox
  • Types of in vitro transporter assay
  • Strengths and limitations of different assay systems
  • Importance of lipid membrane for transporter function

 Who Should Attend?

  • Academic researchers
  • Pharma researchers
  • ADME scientists
  • DMPK scientists
  • Toxicology scientists
  • Drug discovery scientists
Speaker Bio
Joseph Zolnerciks, Ph.D.
Vice-President, Business Development
SOLVO Biotechnology USA, Inc.
Dr. Zolnerciks was awarded his Ph.D. in membrane protein biochemistry from Imperial College London, studying the structure and function of the ABC transporter P-glycoprotein. This was followed by a post-doctoral fellowship in the Deptartment of Pharmaceutics at the University of Washington, Seattle, during which he studied the role of nucleoside transporters in the pharmacokinetic and toxicological profile of nucleoside analog drugs. He then returned to the UK to Queen Mary, University of London to further study efflux transporter biology and work on development of novel assay systems to examine their function. Dr. Zolnerciks joined SOLVO Biotechnology in 2012 as Head of Production, where he was responsible for overseeing membrane- and cell-based product development and manufacture, as well as leading improvements in bioprocess development. In 2014 he moved to the US subsidiary, SOLVO Biotechnology USA, Inc., where he has taken on increasing responsibility for sales and business development, opening the West Coast sales office in Seattle, and currently leading SOLVO's global sales team.